Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 198.8 / NA 48coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/23);  total (25/23)

CTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGC  >  NC_000913/1874919‑1875182
                                                                                                                                    |                                                                                                                                   
cTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac                                                                                                                               <  1:1115436/139‑3 (MQ=255)
cTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac                                                                                                                               <  2:878919/139‑3 (MQ=255)
      ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACa                                                                                                                        >  1:702578/1‑139 (MQ=255)
           aGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGccc                                                                                                                   >  2:629196/1‑139 (MQ=255)
               ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTc                                                                                                               >  1:222587/1‑139 (MQ=255)
                    aTCAGCTGTAACTGCTGCCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcat                                                                                                          <  1:502325/139‑1 (MQ=255)
                     tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                         <  1:107378/139‑1 (MQ=255)
                     tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                         <  2:702578/139‑1 (MQ=255)
                         cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                            <  2:597369/132‑1 (MQ=255)
                         cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                            >  1:597369/1‑132 (MQ=255)
                                   tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                           <  2:905308/139‑1 (MQ=255)
                                    gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                         <  2:678246/124‑1 (MQ=255)
                                    gACGCCACTGGCGAAAACACAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                         >  1:678246/1‑124 (MQ=255)
                                     aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                         <  2:222587/139‑1 (MQ=255)
                                            tGGCGAAACCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATAGTGCGGAtttt                                                                                  >  2:790744/1‑139 (MQ=255)
                                                 aaaaCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCACCGTGCGGATTTTAGGAt                                                                             >  2:1098608/1‑139 (MQ=255)
                                                                    tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                          >  2:553479/1‑139 (MQ=255)
                                                                     ttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGc                                                         <  1:629196/139‑1 (MQ=255)
                                                                     ttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGc                                                         <  1:158051/139‑1 (MQ=255)
                                                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCATCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                     <  1:790744/139‑1 (MQ=255)
                                                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAg                                                                                                                       >  1:1102664/1‑73 (MQ=255)
                                                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAg                                                                                                                       <  2:1102664/73‑1 (MQ=255)
                                                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                          <  1:247631/102‑1 (MQ=255)
                                                                         tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑ACCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                          >  2:247631/1‑102 (MQ=255)
                                                                          tataagagacagaGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtg                                                    >  2:174974/13‑139 (MQ=255)
                                                                           tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt                                                   >  2:24866/1‑139 (MQ=255)
                                                                             aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                         <  2:1028220/83‑1 (MQ=255)
                                                                             aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                         >  1:1028220/1‑83 (MQ=255)
                                                                             aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGa                                                                              >  1:623008/1‑110 (MQ=255)
                                                                             aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGa                                                                              <  2:623008/110‑1 (MQ=255)
                                                                             aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                 >  2:1201511/1‑139 (MQ=255)
                                                                               tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                               >  2:1127564/1‑139 (MQ=255)
                                                                                  ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                                <  2:605952/56‑2 (MQ=255)
                                                                                  ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                                >  1:605952/1‑55 (MQ=255)
                                                                                            cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                                  >  1:459284/1‑139 (MQ=255)
                                                                                             gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAggg                                 >  1:43367/1‑139 (MQ=255)
                                                                                             gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAggg                                 >  2:409825/1‑139 (MQ=255)
                                                                                              ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                                <  1:174974/139‑1 (MQ=255)
                                                                                               tCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg                               <  1:1201511/139‑1 (MQ=255)
                                                                                                 aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGtt                             >  2:560649/1‑139 (MQ=255)
                                                                                                    cATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                            >  1:801612/1‑57 (MQ=255)
                                                                                                    cATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                            <  2:801612/57‑1 (MQ=255)
                                                                                                       cGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGt                                                           >  1:92533/1‑103 (MQ=255)
                                                                                                       cGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGt                                                           <  2:92533/103‑1 (MQ=255)
                                                                                                              aaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc                <  2:71740/139‑1 (MQ=255)
                                                                                                               agccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                            >  1:811239/1‑46 (MQ=255)
                                                                                                               agccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                            <  2:811239/46‑1 (MQ=255)
                                                                                                                 ccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGt                              >  2:761846/1‑122 (MQ=255)
                                                                                                                 ccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGt                              <  1:761846/122‑1 (MQ=255)
                                                                                                                 ccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTg             >  1:980830/1‑139 (MQ=255)
                                                                                                                            ccccAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGc  <  1:553479/139‑1 (MQ=255)
                                                                                                                            ccccAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGc  <  2:643824/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGC  >  NC_000913/1874919‑1875182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A