Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,875,056 |
(C)6→5 |
coding (521/822 nt) |
yeaM ← |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,875,051 | 0 | C | . | 100.0%
| 113.3
/ NA
| 28 | coding (526/822 nt) | yeaM | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (12/16); total (12/16) |
CACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGG > NC_000913/1874917‑1875184
|
cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccc < 2:1173046/139‑1 (MQ=255)
aCTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca < 1:435018/139‑2 (MQ=255)
tCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac > 2:165436/1‑133 (MQ=255)
tCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac < 1:165436/135‑3 (MQ=255)
ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTc > 2:21799/1‑139 (MQ=255)
ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTc > 2:1502773/1‑139 (MQ=255)
cATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCg < 1:1502773/139‑1 (MQ=255)
aaTCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca < 1:211230/139‑1 (MQ=255)
tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc < 1:21799/139‑1 (MQ=255)
tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCCCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc < 1:1017161/139‑1 (MQ=255)
aGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatcat < 2:1452213/139‑1 (MQ=255)
aGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatcat > 1:896790/1‑139 (MQ=255)
cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGAAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCAtct > 2:230152/1‑139 (MQ=255)
tGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCAtctc > 1:548583/1‑139 (MQ=255)
aCTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTccacc > 1:318999/1‑139 (MQ=255)
tGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAtttt > 1:1632/1‑139 (MQ=255)
cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg < 1:1183188/139‑1 (MQ=255)
cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg > 1:1382874/1‑139 (MQ=255)
aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc < 1:627886/77‑1 (MQ=255)
aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc > 2:627886/1‑77 (MQ=255)
cGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg > 1:144092/1‑139 (MQ=255)
tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACa < 2:223311/82‑1 (MQ=255)
tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACa > 1:223311/1‑82 (MQ=255)
ttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGc < 2:1014471/139‑1 (MQ=255)
tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc < 2:659498/87‑1 (MQ=255)
tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc > 1:659498/1‑87 (MQ=255)
tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCaact < 2:1632/139‑4 (MQ=255)
aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg > 2:1081139/1‑139 (MQ=255)
cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccc > 2:1289161/1‑45 (MQ=255)
cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccc < 1:1289161/45‑1 (MQ=255)
cACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGt < 2:318999/139‑1 (MQ=255)
gCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCaaa < 2:896790/139‑1 (MQ=255)
cAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAAc < 1:1081139/139‑1 (MQ=255)
aaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc < 2:123399/139‑1 (MQ=255)
ccccAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGc < 2:1382874/139‑1 (MQ=255)
ccAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCgg < 1:230152/139‑1 (MQ=255)
|
CACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGG > NC_000913/1874917‑1875184
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
