Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 218.9 / NA 54coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/29);  total (25/29)

TTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTG  >  NC_000913/1874914‑1875181
                                                                                                                                         |                                                                                                                                  
tttCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcc                                                                                                                                   <  1:1258771/139‑1 (MQ=255)
    aCTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                               <  2:955568/139‑2 (MQ=255)
          cccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGAc                                                                                                                        <  1:1494668/139‑1 (MQ=255)
           ccTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACa                                                                                                                       >  2:728300/1‑139 (MQ=255)
             tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                    >  2:1613061/1‑125 (MQ=255)
             tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                    <  1:1613061/125‑1 (MQ=255)
             tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAgg                                                                                                                     >  2:1132292/1‑139 (MQ=255)
                  cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCtt                                                                                                                >  2:187953/1‑139 (MQ=255)
                   gcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCttt                                                                                                               <  1:544368/139‑1 (MQ=255)
                    ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTc                                                                                                              >  1:1212874/1‑139 (MQ=255)
                          tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        <  1:969424/139‑1 (MQ=255)
                          tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        <  1:728300/139‑1 (MQ=255)
                             gCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCatcatc                                                                                                     >  1:1381287/1‑139 (MQ=255)
                                      gctgACGCGACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATc                                                                                            >  1:1127820/1‑139 (MQ=255)
                                        tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTTACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                          >  1:1729339/1‑139 (MQ=255)
                                        tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        <  1:557101/125‑1 (MQ=255)
                                        tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                        >  2:557101/1‑125 (MQ=255)
                                          aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTTACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                        <  2:1729339/139‑1 (MQ=255)
                                                    cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                                                              <  1:1705434/139‑1 (MQ=255)
                                                         aCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgag                                                                         <  1:1176208/139‑1 (MQ=255)
                                                            cAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGagaag                                                                      <  2:473907/139‑1 (MQ=255)
                                                                    tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             <  2:1481350/77‑4 (MQ=255)
                                                                    tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             >  1:1481350/1‑74 (MQ=255)
                                                                     ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          <  2:1231999/94‑1 (MQ=255)
                                                                     ctctTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          >  1:1231999/1‑94 (MQ=255)
                                                                         tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                         >  1:1353737/1‑139 (MQ=255)
                                                                         tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                         <  2:1381287/139‑1 (MQ=255)
                                                                             cTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGTCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGc                                                     <  2:1127820/139‑1 (MQ=255)
                                                                             cTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGc                                                     >  1:458963/1‑139 (MQ=255)
                                                                              tATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                    <  2:249590/139‑1 (MQ=255)
                                                                                  aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                >  1:1461245/1‑139 (MQ=255)
                                                                                  aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                >  1:972868/1‑139 (MQ=255)
                                                                                    tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                              >  1:1420714/1‑139 (MQ=255)
                                                                                       ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          <  2:1530343/76‑1 (MQ=255)
                                                                                       ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                          >  1:1530343/1‑76 (MQ=255)
                                                                                       ggCAAGATTGCCTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                           <  1:1132292/139‑1 (MQ=255)
                                                                                        gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           <  1:1263452/74‑1 (MQ=255)
                                                                                        gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           >  2:1263452/1‑74 (MQ=255)
                                                                                        gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTgg                                          <  2:1353737/139‑1 (MQ=255)
                                                                                                 cGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                                 <  1:187953/139‑1 (MQ=255)
                                                                                                     cACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGt                             <  2:1420714/139‑1 (MQ=255)
                                                                                                      aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               <  1:729797/136‑1 (MQ=255)
                                                                                                      aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               >  2:729797/1‑136 (MQ=255)
                                                                                                       cTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtc                           >  2:1088282/1‑139 (MQ=255)
                                                                                                          aTCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtcatc                        >  2:367943/1‑139 (MQ=255)
                                                                                                           tCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             <  1:1337533/38‑4 (MQ=255)
                                                                                                           tCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccact                                                                                                                             >  2:1337533/1‑35 (MQ=255)
                                                                                                            cGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                         <  2:800956/72‑1 (MQ=255)
                                                                                                            cGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                         >  1:800956/1‑72 (MQ=255)
                                                                                                                aaaaaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                                                          >  2:1517961/1‑83 (MQ=255)
                                                                                                                aaaaaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATga                                                                          <  1:1517961/83‑1 (MQ=255)
                                                                                                                   aaGCCAGCCCATTGCCCCAATG‑TCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc               <  2:1212874/139‑1 (MQ=255)
                                                                                                                    agccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           >  2:119761/1‑46 (MQ=39)
                                                                                                                    agccagccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                           <  1:119761/46‑1 (MQ=39)
                                                                                                                         gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                    >  2:618517/1‑96 (MQ=255)
                                                                                                                         gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                    <  1:618517/96‑1 (MQ=255)
                                                                                                                           ccATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTa       <  2:458963/139‑1 (MQ=255)
                                                                                                                               tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               >  1:1543995/1‑111 (MQ=255)
                                                                                                                               tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                               <  2:1543995/111‑1 (MQ=255)
                                                                                                                               tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCt   >  1:324442/1‑139 (MQ=255)
                                                                                                                                gCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTg  <  2:1461245/139‑1 (MQ=255)
                                                                                                                                gCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATAATCTCCACCTTCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTg  <  1:1088282/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
TTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTG  >  NC_000913/1874914‑1875181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A