Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,056 (C)6→5 coding (521/822 nt) yeaM ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,0510C.100.0% 211.8 / NA 50coding (526/822 nt)yeaMputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (25/25);  total (25/25)

CTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCT  >  NC_000913/1874913‑1875180
                                                                                                                                          |                                                                                                                                 
cTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGc                                                                                                                                   <  2:407403/139‑1 (MQ=255)
     aCTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                              <  2:521953/139‑2 (MQ=255)
      cTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcccccac                                                                                                                             <  2:13988/139‑3 (MQ=255)
          tCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGa                                                                                                                        >  1:423941/1‑139 (MQ=255)
              tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAgg                                                                                                                    >  1:737981/1‑139 (MQ=255)
                    gcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCttt                                                                                                              <  2:423941/139‑1 (MQ=255)
                        tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                          <  2:988367/139‑1 (MQ=255)
                        tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGcc                                                                                                          <  1:541899/139‑1 (MQ=255)
                          aTCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcat                                                                                                        <  2:427335/139‑1 (MQ=255)
                          aTCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcat                                                                                                        <  1:447813/139‑1 (MQ=255)
                               cTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCAtct                                                                                                   >  2:342178/1‑139 (MQ=255)
                                  tAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                              <  1:1057516/110‑2 (MQ=255)
                                  tAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                              >  2:1057516/1‑109 (MQ=255)
                                     ctgctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTccacca                                                                                             <  2:370798/139‑1 (MQ=255)
                                         tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                              <  1:1008375/103‑2 (MQ=255)
                                         tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGccccca                                                                                                                              >  2:1008375/1‑102 (MQ=255)
                                         tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                         >  2:37471/1‑139 (MQ=255)
                                          gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                        >  2:843237/1‑139 (MQ=255)
                                           aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                       <  1:37471/139‑1 (MQ=255)
                                                     cGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGTGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAgg                                                                             <  1:649437/139‑1 (MQ=255)
                                                      gAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGa                                                                            <  1:843237/139‑1 (MQ=255)
                                                              aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       <  1:564906/104‑1 (MQ=255)
                                                              aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       >  2:564906/1‑104 (MQ=255)
                                                                              cTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGc                                                    <  2:737981/139‑1 (MQ=255)
                                                                                 tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt                                                 >  2:1026914/1‑139 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAg                                                                                                                     <  1:990345/69‑1 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAg                                                                                                                     >  2:990345/1‑69 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCg                                                                                                            >  1:507125/1‑78 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCg                                                                                                            <  2:507125/78‑1 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       >  2:321640/1‑83 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       <  1:321640/83‑1 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                       <  1:422911/99‑1 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                       >  2:422911/1‑99 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                               >  1:348706/1‑139 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                               >  2:53867/1‑139 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                               >  1:79159/1‑139 (MQ=255)
                                                                                   aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                               >  2:68390/1‑139 (MQ=255)
                                                                                     tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                             >  2:501772/1‑139 (MQ=255)
                                                                                     tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                             >  2:990499/1‑139 (MQ=255)
                                                                                        ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACCGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                          <  2:768798/139‑1 (MQ=255)
                                                                                        ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                          <  2:348706/139‑1 (MQ=255)
                                                                                         gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCgg                                                                                     <  2:55394/95‑1 (MQ=255)
                                                                                         gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCgg                                                                                     >  1:55394/1‑95 (MQ=255)
                                                                                           aaGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                         >  1:959467/1‑73 (MQ=255)
                                                                                           aaGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCca                                                                                                         <  2:959467/73‑1 (MQ=255)
                                                                                                    ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                              <  1:342178/139‑1 (MQ=255)
                                                                                                        cTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                        >  2:231435/1‑109 (MQ=255)
                                                                                                        cTCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                        <  1:231435/109‑1 (MQ=255)
                                                                                                         tCATCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtca                         >  2:671883/1‑139 (MQ=255)
                                                                                                            tCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       >  1:754535/1‑58 (MQ=255)
                                                                                                            tCGCAAAAAAGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCcatc                                                                                                       <  2:754535/58‑1 (MQ=255)
                                                                                                                    aaGCCAGCCCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc              <  1:53867/139‑1 (MQ=255)
                                                                                                                          gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                                <  1:193543/115‑1 (MQ=255)
                                                                                                                          gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAgg                                >  2:193543/1‑115 (MQ=255)
                                                                                                                          gccCATTGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc              >  1:584137/1‑133 (MQ=255)
                                                                                                                          gccCATTGACCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc              <  2:584137/133‑1 (MQ=255)
                                                                                                                                tGCCCCAATG‑CCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCt  >  2:220596/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                 
CTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCCCCCCACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCT  >  NC_000913/1874913‑1875180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A