Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,939,673	T→C	N174S (AAC→AGC)	lpxM ←	myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,939,673	0	T	C	100.0%	147.3 / NA	41	N174S (AAC→AGC)	lpxM	myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/22); total (19/22)

TGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAAC  >  NC_000913/1939565‑1939797

tGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACa                                                                                                                      <  2:522051/117‑1 (MQ=255)

tGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACa                                                                                                                      >  1:522051/1‑117 (MQ=255)

 gATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                                   <  1:1218310/135‑1 (MQ=255)

 gATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                                   >  2:1218310/1‑135 (MQ=255)

       gTAAATAATATCCCCGGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATg                                                                                         >  1:813937/1‑139 (MQ=255)

       gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                  >  2:950370/1‑130 (MQ=255)

       gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                  <  1:950370/130‑1 (MQ=255)

        tAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCACATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATgg                                                                                        >  2:350580/1‑139 (MQ=255)

               tatCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCACATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                 <  1:350580/139‑1 (MQ=255)

                 tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                               >  2:85474/1‑139 (MQ=255)

                         aCCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAtttt                                                                       <  1:85474/139‑1 (MQ=255)

                           cccTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCt                                                                     >  2:558094/1‑139 (MQ=255)

                                    aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                            <  2:662014/139‑1 (MQ=255)

                                     ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGa                                                                                              >  1:1097505/1‑104 (MQ=255)

                                     ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGa                                                                                              <  2:1097505/104‑1 (MQ=255)

                                      cGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAg                                                          <  1:1012150/139‑1 (MQ=255)

                                          tGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGcc                                                       >  1:120749/1‑138 (MQ=255)

                                          tGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGcc                                                       <  2:120749/138‑1 (MQ=255)

                                             aTGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCa                                                   <  2:813937/139‑1 (MQ=255)

                                              tGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAg                                                  <  2:160883/139‑1 (MQ=255)

                                               gAATGGATTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGc                                                 >  1:590860/1‑139 (MQ=255)

                                                  tGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATg                                              <  1:303227/139‑1 (MQ=255)

                                                  tGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATg                                              <  2:483110/139‑1 (MQ=255)

                                                     tttAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCa                                           <  2:590860/139‑1 (MQ=255)

                                                             cGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAGAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                            >  2:723442/1‑114 (MQ=255)

                                                             cGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATGATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                            <  1:723442/114‑1 (MQ=255)

                                                               tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                 >  2:630134/1‑91 (MQ=255)

                                                               tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                 <  1:630134/91‑1 (MQ=255)

                                                               tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                 >  1:383044/1‑139 (MQ=255)

                                                                       cGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGc                                                 <  2:582909/115‑1 (MQ=255)

                                                                       cGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGc                                                 >  1:582909/1‑115 (MQ=255)

                                                                           tGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGt                     >  2:887673/1‑139 (MQ=255)

                                                                            gCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg                    <  2:352148/139‑1 (MQ=255)

                                                                             cAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGc                   >  1:827139/1‑139 (MQ=255)

                                                                               gacgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCgg                 <  1:558094/139‑1 (MQ=255)

                                                                                acgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                       <  2:176092/52‑1 (MQ=255)

                                                                                acgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                       >  1:176092/1‑52 (MQ=255)

                                                                                    ccGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCa            >  2:731031/1‑139 (MQ=255)

                                                                                    ccGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCa            >  2:943679/1‑139 (MQ=255)

                                                                                     cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAg           <  2:383044/139‑1 (MQ=255)

                                                                                              gacgacGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAAc  <  1:887673/139‑1 (MQ=255)

TGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAAC  >  NC_000913/1939565‑1939797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A