Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,939,673 T→C N174S (AAC→AGC)  lpxM ← myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,939,6730TC100.0% 253.2 / NA 68N174S (AAC→AGC) lpxMmyristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

CGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGA  >  NC_000913/1939542‑1939789
                                                                                                                                   |                                                                                                                    
cGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGAc                                                                                                               >  2:28829/1‑139 (MQ=255)
                 tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                               <  1:840020/138‑1 (MQ=255)
                 tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                               >  2:840020/1‑138 (MQ=255)
                 tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGt                                                                                              >  1:277761/1‑139 (MQ=255)
                    tCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                           <  2:33236/139‑1 (MQ=255)
                     ccTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          <  2:984970/139‑1 (MQ=255)
                      cTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGccc                                                                                         <  2:277761/139‑1 (MQ=255)
                       tGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                        <  1:22208/139‑1 (MQ=255)
                       tGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                        <  1:28829/139‑1 (MQ=255)
                              gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATg                                                                                 >  1:1127120/1‑139 (MQ=255)
                                       atCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATa                                                                                                            <  2:613002/103‑1 (MQ=255)
                                       atCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATa                                                                                                            >  1:613002/1‑103 (MQ=255)
                                        tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                       >  2:779409/1‑139 (MQ=255)
                                             aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                           <  1:972938/114‑1 (MQ=255)
                                             aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                           >  2:972938/1‑114 (MQ=255)
                                             aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAt                                                                  >  1:472958/1‑139 (MQ=255)
                                             aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAt                                                                  >  1:848424/1‑139 (MQ=255)
                                              gTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACAt                                                                                                             <  2:660532/95‑1 (MQ=255)
                                              gTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACAt                                                                                                             >  1:660532/1‑95 (MQ=255)
                                              gTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                               >  2:356565/1‑109 (MQ=255)
                                              gTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                               <  1:356565/109‑1 (MQ=255)
                                                  cccTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCt                                                             >  1:937189/1‑139 (MQ=255)
                                                    cTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGc                                                           >  1:495518/1‑139 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATc                                                                                                         >  1:561606/1‑86 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATc                                                                                                         <  2:561606/86‑1 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                               >  1:503947/1‑96 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                         <  1:1358699/118‑1 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                         >  2:1358699/1‑118 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                    >  2:732866/1‑139 (MQ=255)
                                                           aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                    <  2:1187425/139‑1 (MQ=255)
                                                           aCCGACTGGATGAATGGTGTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                               <  2:503947/96‑1 (MQ=255)
                                                            ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          <  2:1177708/100‑1 (MQ=255)
                                                            ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          >  1:1177708/1‑100 (MQ=255)
                                                            ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGa                                                   >  2:207715/1‑139 (MQ=255)
                                                             cGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAg                                                  <  2:495518/139‑1 (MQ=255)
                                                                cTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGcc                                               <  1:779409/139‑1 (MQ=255)
                                                                    acgAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCa                                           >  2:1372089/3‑139 (MQ=255)
                                                                      gAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                        >  2:801672/1‑92 (MQ=255)
                                                                      gAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                        <  1:801672/92‑1 (MQ=255)
                                                                                      tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCgg                                                                                                <  2:81711/68‑1 (MQ=255)
                                                                                      tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCgg                                                                                                >  1:81711/1‑68 (MQ=255)
                                                                                      tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          >  1:1354963/1‑74 (MQ=255)
                                                                                      tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          <  2:1354963/74‑1 (MQ=255)
                                                                                      tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGc                                    <  1:1230979/128‑1 (MQ=255)
                                                                                      tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGc                                    >  2:1230979/1‑128 (MQ=255)
                                                                                         tttCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACgg                      <  1:732866/139‑1 (MQ=255)
                                                                                           tctcGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGcc                    <  1:207715/139‑1 (MQ=255)
                                                                                                   gCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg            <  2:472958/139‑1 (MQ=255)
                                                                                                    cAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          <  2:601227/60‑1 (MQ=255)
                                                                                                    cAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                          >  1:601227/1‑60 (MQ=255)
                                                                                                         gacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATc                           >  1:1665212/1‑118 (MQ=255)
                                                                                                         gacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATc                           <  2:1665212/118‑1 (MQ=255)
                                                                                                          acCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCAcc     <  2:1127120/139‑1 (MQ=255)
                                                                                                           ccGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCa                                           >  2:896822/1‑100 (MQ=255)
                                                                                                           ccGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCa                                           <  1:896822/100‑1 (MQ=255)
                                                                                                            cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAg   >  2:665628/1‑139 (MQ=255)
                                                                                                            cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAg   <  1:1372089/139‑1 (MQ=255)
                                                                                                             gCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGa  <  1:390746/139‑1 (MQ=255)
                                                                                                              ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                    >  2:1357657/1‑88 (MQ=255)
                                                                                                              ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCg                                                    <  1:1357657/88‑1 (MQ=255)
                                                                                                               cAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCAt                                             >  1:480235/1‑94 (MQ=255)
                                                                                                               cAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCAt                                             <  2:480235/94‑1 (MQ=255)
                                                                                                                      acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                        >  1:1670817/1‑44 (MQ=255)
                                                                                                                      acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                        <  2:1670817/44‑1 (MQ=255)
                                                                                                                      acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAg                                          >  2:954438/1‑90 (MQ=255)
                                                                                                                      acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAg                                          <  1:954438/90‑1 (MQ=255)
                                                                                                                      acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCC‑GATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAg                                          <  1:861323/89‑1 (MQ=255)
                                                                                                                      acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCC‑GATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAg                                          >  2:861323/1‑89 (MQ=255)
                                                                                                                                   |                                                                                                                    
CGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGA  >  NC_000913/1939542‑1939789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A