Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,939,673	T→C	N174S (AAC→AGC)	lpxM ←	myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,939,673	0	T	C	100.0%	194.3 / NA	55	N174S (AAC→AGC)	lpxM	myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/25); total (30/25)

ATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTA  >  NC_000913/1939539‑1939807

aTTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCa                                                                                                                                    >  1:547476/1‑139 (MQ=255)

                  cATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCgg                                                                                                                  >  1:363360/1‑139 (MQ=255)

                    tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                                 <  1:1009048/138‑1 (MQ=255)

                    tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                                 >  2:1009048/1‑138 (MQ=255)

                    tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGt                                                                                                                >  1:457262/1‑139 (MQ=255)

                        ccTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  2:547476/139‑1 (MQ=255)

                                ggTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTAt                                                                                                    >  2:973120/1‑139 (MQ=255)

                                 gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGa                                                                                                        >  1:527679/1‑134 (MQ=255)

                                 gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGa                                                                                                        <  2:527679/134‑1 (MQ=255)

                                      taataTCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACa                                                                                              >  1:187749/1‑139 (MQ=255)

                                         tatCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGTAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                           >  2:862223/1‑139 (MQ=255)

                                         tatCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                           <  2:363360/139‑1 (MQ=255)

                                           tCCCCAGTACCCCTGACGTACCGACTGGCTGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                                         >  1:703144/1‑139 (MQ=255)

                                           tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAACCCGGGTTGCCCTGATTATGGAACATCGCt                                                                                         >  2:193051/1‑139 (MQ=255)

                                           tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                           <  1:271651/137‑1 (MQ=255)

                                           tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCg                                                                                           >  2:271651/1‑137 (MQ=255)

                                               cAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCa                                                                                     >  1:810628/1‑139 (MQ=255)

                                                 gTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCTACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCAtt                                                                                   <  2:187749/139‑1 (MQ=255)

                                                     cccTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  1:538744/110‑1 (MQ=255)

                                                     cccTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  2:538744/1‑110 (MQ=255)

                                                      ccTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGTAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTg                                                                              <  1:862223/139‑1 (MQ=255)

                                                       cTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGc                                                                             <  1:862238/139‑1 (MQ=255)

                                                       cTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGc                                                                             >  1:82387/1‑139 (MQ=255)

                                                       cTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGc                                                                             >  2:769572/1‑139 (MQ=255)

                                                             tACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGc                                                                       <  1:769572/139‑1 (MQ=255)

                                                              aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  2:821638/101‑1 (MQ=255)

                                                              aCCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  1:821638/1‑101 (MQ=255)

                                                               ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                                 >  1:430239/1‑95 (MQ=255)

                                                               ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                                 <  2:430239/95‑1 (MQ=255)

                                                               ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGa                                                                     >  1:1118310/1‑139 (MQ=255)

                                                                 gACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGGCCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  2:802723/1‑98 (MQ=255)

                                                                 gACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  1:802723/98‑1 (MQ=255)

                                                                 gACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  1:1119684/98‑1 (MQ=255)

                                                                 gACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  2:1119684/1‑98 (MQ=255)

                                                                   cTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGcc                                                                 <  1:67437/139‑1 (MQ=255)

                                                                     ggATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCAt                                                               <  1:973120/139‑1 (MQ=255)

                                                                                     aCCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAata                                               >  2:642170/1‑139 (MQ=255)

                                                                                        gTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCAt                                                               <  2:1104867/120‑1 (MQ=255)

                                                                                        gTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCAt                                                               >  1:1104867/1‑120 (MQ=255)

                                                                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                           >  1:536583/1‑139 (MQ=255)

                                                                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                           >  2:86492/1‑139 (MQ=255)

                                                                                         tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                           >  1:1053195/1‑139 (MQ=255)

                                                                                                 cGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCaa                                   <  1:642170/139‑1 (MQ=255)

                                                                                                   cATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCTGCATGGCAGGAATATCGACGGCCCAAcc                                 >  1:680719/1‑139 (MQ=255)

                                                                                                   cATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAAcc                                 >  2:266431/1‑139 (MQ=255)

                                                                                                      gCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg                              <  2:536583/139‑1 (MQ=255)

                                                                                                               cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg                              <  1:1026402/130‑1 (MQ=255)

                                                                                                               cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg                              >  2:1026402/1‑130 (MQ=255)

                                                                                                                 ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGTCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg                              <  1:987892/128‑1 (MQ=255)

                                                                                                                 ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTg                              >  2:987892/1‑128 (MQ=255)

                                                                                                                           gacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCGGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACttt         <  2:680719/139‑1 (MQ=255)

                                                                                                                            acgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCa                                                     <  2:986247/94‑1 (MQ=255)

                                                                                                                            acgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCa                                                     >  1:986247/1‑94 (MQ=255)

                                                                                                                             cgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTtct       <  2:1053195/139‑1 (MQ=255)

                                                                                                                                  cGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGtta  <  2:457262/139‑1 (MQ=255)

ATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTA  >  NC_000913/1939539‑1939807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A