Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,532	T→C	D260G (GAT→GGT)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,532	0	T	C	100.0%	160.7 / NA	46	D260G (GAT→GGT)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/22); total (24/22)

ATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGAT  >  NC_000913/4634407‑4634667

aTCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATTAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCTTGACCTTTACg                                                                                                                            >  1:32177/1‑139 (MQ=255)

 tCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACgg                                                                                                                           <  1:1012403/139‑1 (MQ=255)

          cccTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAgg                                                                                                                  >  1:477695/1‑139 (MQ=255)

            cTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGtt                                                                                                                >  2:572248/1‑139 (MQ=255)

             tGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTg                                                                                                               >  2:110759/1‑139 (MQ=255)

                       gAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCa                                                                                                     <  2:249980/139‑1 (MQ=255)

                       gAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCa                                                                                                     <  1:195758/139‑1 (MQ=255)

                         gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTc                                                                                                                     <  2:747873/121‑1 (MQ=255)

                         gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTc                                                                                                                     >  1:747873/1‑121 (MQ=255)

                         gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                   >  2:739286/1‑139 (MQ=255)

                         gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                   >  2:290896/1‑139 (MQ=255)

                         gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCACTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                   >  2:657972/1‑139 (MQ=255)

                            gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                                >  2:808726/1‑139 (MQ=255)

                            gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                                <  1:450864/139‑1 (MQ=255)

                            gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCACTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                                <  1:657972/139‑1 (MQ=255)

                             cAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACg                                                                                               >  1:517796/1‑139 (MQ=255)

                                           gATCTGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGc                                                                                                  <  2:221838/122‑1 (MQ=255)

                                           gATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGc                                                                                                  >  1:221838/1‑122 (MQ=255)

                                            aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCa                                                                                >  1:637811/1‑139 (MQ=255)

                                                          ttCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTGACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGc                                                                  >  1:770609/1‑139 (MQ=255)

                                                               aGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                             <  2:477695/139‑1 (MQ=255)

                                                                    aGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTc                                                        >  1:1179248/1‑139 (MQ=255)

                                                                     gATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTcc                                                       <  2:517796/139‑1 (MQ=255)

                                                                      aTTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTccc                                                      >  1:413358/1‑139 (MQ=255)

                                                                           ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                 <  2:637811/139‑1 (MQ=255)

                                                                           ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                 >  2:1039414/1‑139 (MQ=255)

                                                                           ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                 >  1:117765/1‑139 (MQ=255)

                                                                             gggCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCtt                                               <  2:413358/139‑1 (MQ=255)

                                                                                cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                            >  2:546071/1‑139 (MQ=255)

                                                                                cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                            >  2:999865/1‑139 (MQ=255)

                                                                                    ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:999865/139‑1 (MQ=255)

                                                                                    ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:572248/139‑1 (MQ=255)

                                                                                    ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:290896/139‑1 (MQ=255)

                                                                                    ctcCCGCTTTGGCATCTTCGGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:92083/139‑1 (MQ=255)

                                                                                    ctaaggatatGGCATATTCTGCCGGGTAGTCCCGCCCAATCCCCGGAACATTCCGGCGCGTCAGGTTGAGCATTGGCATGCACGATCCCTATACCGTCAGGATATACTCCTGCACGCCGGTTCCCAGACCCTCATAGGt                                        <  1:608331/130‑1 (MQ=255)

                                                                                       ccGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaaa                                     <  1:110759/139‑1 (MQ=255)

                                                                                          cTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGtt                                                                                             <  2:1045285/80‑1 (MQ=255)

                                                                                          cTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGtt                                                                                             >  1:1045285/1‑80 (MQ=255)

                                                                                              ggCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                             <  2:1050916/108‑1 (MQ=255)

                                                                                              ggCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                             >  1:1050916/1‑108 (MQ=255)

                                                                                               gCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCtt                                               <  2:517699/121‑1 (MQ=255)

                                                                                               gCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCtt                                               >  1:517699/1‑121 (MQ=255)

                                                                                                    ttctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAg                                                    <  2:971498/111‑1 (MQ=255)

                                                                                                    ttctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAg                                                    >  1:971498/1‑111 (MQ=255)

                                                                                                     tctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcgc                       <  2:770609/139‑1 (MQ=255)

                                                                                                                gtaTCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgcagc            >  2:710623/1‑139 (MQ=255)

                                                                                                                          atacCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGAt  >  1:1019445/1‑139 (MQ=255)

ATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGAT  >  NC_000913/4634407‑4634667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A