Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	762,739	G→A	406 (TAG→TAA)	sucB →	dihydrolipoyltranssuccinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	762,739	0	G	A	100.0%	152.9 / NA	48	406 (TAG→TAA)	sucB	dihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/25); total (23/25)

GCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGA  >  NC_000913/762616‑762860

gCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                            <  1:1606311/139‑1 (MQ=255)

                 cgcTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGa                                                                                           <  1:422152/139‑1 (MQ=255)

                                aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                         <  1:177804/110‑1 (MQ=255)

                                aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                         >  2:177804/1‑110 (MQ=255)

                                aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAG‑TTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCt                                                                           >  2:652534/1‑139 (MQ=255)

                                aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCAGCTGCTGGACGTGTAATGGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGcc                                                                            >  1:1075559/1‑139 (MQ=255)

                                aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGAACAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGAt                                                                                          <  1:672889/125‑1 (MQ=255)

                                aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGAACAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGAt                                                                                          >  2:672889/1‑125 (MQ=255)

                                      tGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTcc                                                                      <  1:407165/139‑1 (MQ=255)

                                        aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                               <  2:914643/112‑1 (MQ=255)

                                        aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                               >  1:914643/1‑112 (MQ=255)

                                                  gcgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCAc                                                                                                      >  1:1618262/1‑95 (MQ=255)

                                                  gcgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCAc                                                                                                      <  2:1618262/95‑1 (MQ=255)

                                                  gcgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGc                                                          >  1:20903/1‑139 (MQ=255)

                                                   cgAGTCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCAGTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGcc                                                         >  1:1075552/1‑139 (MQ=255)

                                                   cgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGcc                                                         <  2:20903/139‑1 (MQ=255)

                                                       tCCGTGGGCTTCCTGGTAACGATCAATGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAG‑TTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATg                                                    <  1:652534/139‑1 (MQ=255)

                                                                 tCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                           <  2:379026/139‑1 (MQ=255)

                                                                  ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGTTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCg                                                                              >  1:959341/1‑103 (MQ=255)

                                                                  ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGTTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCg                                                                              <  2:959341/103‑1 (MQ=255)

                                                                  ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGAc                                          >  1:1225648/1‑139 (MQ=255)

                                                                            aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGtt                                                                                                                      <  1:141030/53‑1 (MQ=255)

                                                                            aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGtt                                                                                                                      >  2:141030/1‑53 (MQ=255)

                                                                            aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                         >  1:1337242/1‑66 (MQ=255)

                                                                            aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                         <  2:1337242/66‑1 (MQ=255)

                                                                            aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATc                                >  1:1073429/1‑139 (MQ=255)

                                                                              cAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                              <  2:1075559/139‑1 (MQ=255)

                                                                                aaGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                              <  1:1281705/137‑1 (MQ=255)

                                                                                aaGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                              >  2:1281705/1‑137 (MQ=255)

                                                                                aaGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGc                            >  2:734796/1‑139 (MQ=255)

                                                                                     ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                <  2:166550/66‑1 (MQ=255)

                                                                                     ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                >  1:166550/1‑66 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                         <  2:1141811/55‑1 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                         >  1:1141811/1‑55 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                           <  2:574613/117‑1 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                           >  1:574613/1‑117 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                     <  2:1075552/139‑1 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                     <  2:1073429/139‑1 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                     >  1:385566/1‑139 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                     <  1:734796/139‑1 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg                      >  1:207121/1‑138 (MQ=255)

                                                                                       gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg                      <  2:207121/138‑1 (MQ=255)

                                                                                           gAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGacca                 <  2:385566/139‑1 (MQ=255)

                                                                                                  cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGaa                                                            <  1:847400/89‑1 (MQ=255)

                                                                                                  cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGaa                                                            >  2:847400/1‑89 (MQ=255)

                                                                                                          ctgctgctgGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGa  >  1:989980/1‑139 (MQ=255)

                                                                                                                       tgtAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGac                   >  2:978090/1‑109 (MQ=255)

                                                                                                                       tgtAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGac                   <  1:978090/109‑1 (MQ=255)

GCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGA  >  NC_000913/762616‑762860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A