Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	762,739	G→A	406 (TAG→TAA)	sucB →	dihydrolipoyltranssuccinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	762,739	0	G	A	100.0%	202.6 / NA	62	406 (TAG→TAA)	sucB	dihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/32); total (30/32)

AGATCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCG  >  NC_000913/762609‑762873

agaTCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTaa                                                                                                                                >  2:1371119/1‑139 (MQ=255)

  aTCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                              <  1:171827/139‑1 (MQ=255)

  aTCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                              <  1:168983/139‑1 (MQ=255)

             gatgTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCAc                                                                                                                   >  1:589690/1‑139 (MQ=255)

                   ccTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                             <  2:616316/139‑1 (MQ=255)

                        cgcTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACt                                                                                                                  >  1:1747129/1‑129 (MQ=255)

                        cgcTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACt                                                                                                                  <  2:1747129/129‑1 (MQ=255)

                        cgcTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGa                                                                                                        <  2:382941/139‑1 (MQ=255)

                                       aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                              >  2:144682/1‑102 (MQ=255)

                                       aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                              <  1:144682/102‑1 (MQ=255)

                                       aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                                            >  2:1515537/1‑120 (MQ=255)

                                       aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                                            <  1:1515537/120‑1 (MQ=255)

                                       aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGcc                                                                                         >  2:529852/1‑139 (MQ=255)

                                       aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGcc                                                                                         >  1:80388/1‑139 (MQ=255)

                                         cGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCtt                                                                                       >  1:221356/1‑139 (MQ=255)

                                          gTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                         <  2:1350184/104‑1 (MQ=255)

                                          gTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                         >  1:1350184/1‑104 (MQ=255)

                                               aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCgg                                                                                 <  2:589690/139‑1 (MQ=255)

                                               aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCgg                                                                                 <  2:80388/139‑1 (MQ=255)

                                                tCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                             >  1:412424/1‑110 (MQ=255)

                                                tCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                             <  2:412424/110‑1 (MQ=255)

                                                tCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGc                                                                                <  1:935554/139‑1 (MQ=255)

                                                                    ggCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACg                                                            <  1:1568938/139‑1 (MQ=255)

                                                                        tCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                                        <  1:1371119/139‑1 (MQ=255)

                                                                        tCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                                        <  1:529852/139‑1 (MQ=255)

                                                                         ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                         >  2:1062416/1‑73 (MQ=255)

                                                                         ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                         <  1:1062416/73‑1 (MQ=255)

                                                                         ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg                                                                                                    >  1:1577851/1‑94 (MQ=255)

                                                                         ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg                                                                                                    <  2:1577851/94‑1 (MQ=255)

                                                                         ccTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGAc                                                       >  2:1193253/1‑139 (MQ=255)

                                                                                   aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACt                                                                                                                  >  2:443559/1‑70 (MQ=255)

                                                                                   aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACt                                                                                                                  <  1:443559/70‑1 (MQ=255)

                                                                                   aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATc                                             <  2:221356/139‑1 (MQ=255)

                                                                                   aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACC‑GATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCa                                            >  1:1337931/1‑139 (MQ=255)

                                                                                   aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGA‑GTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCa                                            >  1:1598066/1‑139 (MQ=255)

                                                                                    tCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCa                                            >  2:665319/1‑139 (MQ=255)

                                                                                     cAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATg                                                                 <  1:1444365/117‑1 (MQ=255)

                                                                                     cAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATg                                                                 >  2:1444365/1‑117 (MQ=255)

                                                                                            ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                                      <  1:1405110/57‑1 (MQ=255)

                                                                                            ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg                                                                                                                      >  2:1405110/1‑57 (MQ=255)

                                                                                             tGCTGGAAGATCCGACGCGTCTGCTGCTGGA‑GTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  <  2:1598066/139‑1 (MQ=255)

                                                                                              gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                                        <  2:1007077/117‑1 (MQ=255)

                                                                                              gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                                        >  1:1007077/1‑117 (MQ=255)

                                                                                              gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  >  2:84852/1‑139 (MQ=255)

                                                                                              gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  <  2:1656166/139‑1 (MQ=255)

                                                                                                    aGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                              >  1:1012881/1‑41 (MQ=255)

                                                                                                    aGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                              <  2:1012881/41‑1 (MQ=255)

                                                                                                    aGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  <  2:220950/133‑1 (MQ=255)

                                                                                                    aGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  >  1:220950/1‑133 (MQ=255)

                                                                                                         cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                             >  2:331626/1‑53 (MQ=255)

                                                                                                         cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa                                                                                                             <  1:331626/53‑1 (MQ=255)

                                                                                                         cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGAcg                                                      >  1:1397924/1‑108 (MQ=255)

                                                                                                         cGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGAcg                                                      <  2:1397924/108‑1 (MQ=255)

                                                                                                           aCGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAg                     <  1:84852/139‑1 (MQ=255)

                                                                                                            cgcgTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  <  1:757511/125‑1 (MQ=255)

                                                                                                            cgcgTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                  >  2:757511/1‑125 (MQ=255)

                                                                                                                 ctgctgctgGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGa               >  1:1146925/1‑139 (MQ=255)

                                                                                                                          gACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCa      <  1:1193253/139‑1 (MQ=255)

                                                                                                                            cGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAg    >  1:786799/1‑139 (MQ=255)

                                                                                                                              tgtAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                                        >  1:1250465/1‑85 (MQ=255)

                                                                                                                              tgtAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGa                                                        <  2:1250465/85‑1 (MQ=255)

                                                                                                                              tgtAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAgcg  >  2:1711488/1‑139 (MQ=255)

AGATCCTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCG  >  NC_000913/762609‑762873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A