Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
762,739 |
G→A |
*406* (TAG→TAA) |
sucB → |
dihydrolipoyltranssuccinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 762,739 | 0 | G | A | 100.0%
| 88.1
/ NA
| 28 | *406* (TAG→TAA) | sucB | dihydrolipoyltranssuccinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/15); total (13/15) |
GCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGG > NC_000913/762632‑762841
|
gcgcTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCgg > 2:832713/1‑139 (MQ=255)
aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg < 2:777250/110‑1 (MQ=255)
aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg > 1:777250/1‑110 (MQ=255)
aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCa > 2:733941/1‑104 (MQ=255)
aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCa < 1:733941/104‑1 (MQ=255)
aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa > 2:298090/1‑111 (MQ=255)
aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGa < 1:298090/111‑1 (MQ=255)
aTCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCgg < 1:832713/139‑1 (MQ=255)
aTGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAAt > 1:466691/1‑139 (MQ=255)
tCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAAtt < 1:300294/136‑1 (MQ=255)
tCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAAtt > 2:300294/1‑136 (MQ=255)
cgAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGcc < 2:110457/139‑1 (MQ=255)
tGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg > 1:463030/1‑101 (MQ=255)
tGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg < 2:463030/101‑1 (MQ=255)
tGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGAcgc < 2:148839/139‑1 (MQ=255)
aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGt < 2:145858/133‑1 (MQ=255)
aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGt > 1:145858/1‑133 (MQ=255)
aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATc > 2:783727/1‑139 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc > 2:224435/1‑52 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc < 1:224435/52‑1 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg < 1:205592/55‑1 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTg > 2:205592/1‑55 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:8683/130‑1 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg > 2:8683/1‑130 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg < 2:637636/139‑1 (MQ=255)
gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg < 2:466691/139‑1 (MQ=255)
aGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCAtt < 2:749097/72‑1 (MQ=255)
aGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCAtt > 1:749097/1‑72 (MQ=255)
|
GCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGG > NC_000913/762632‑762841
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
