Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	762,739	G→A	406 (TAG→TAA)	sucB →	dihydrolipoyltranssuccinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	762,739	0	G	A	100.0%	113.4 / NA	36	406 (TAG→TAA)	sucB	dihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/15); total (21/15)

CTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGC  >  NC_000913/762614‑762874

cTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTc                                                                                                                            <  1:396139/139‑1 (MQ=255)

 tGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCa                                                                                                                           >  1:53918/1‑139 (MQ=255)

 tGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCa                                                                                                                           >  1:553314/1‑139 (MQ=255)

  gCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                          >  2:138960/1‑139 (MQ=255)

    cGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCt                                                                                                                        <  1:578702/139‑1 (MQ=255)

                  gcgcTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGATGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCgg                                                                                                          >  2:535464/1‑139 (MQ=255)

                       gTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg                                                                                                     >  2:737081/1‑139 (MQ=255)

                       gTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAg                                                                                                     >  1:295275/1‑139 (MQ=255)

                        tCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                               <  1:221105/112‑1 (MQ=255)

                        tCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGt                                                                                                                               >  2:221105/1‑112 (MQ=255)

                                  aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGcc                                                                                          >  1:738500/1‑139 (MQ=255)

                                  aCCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGcc                                                                                          >  2:391171/1‑139 (MQ=255)

                                      tCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTtct                                                                                      >  1:213298/1‑139 (MQ=255)

                                       cTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTGACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTtctc                                                                                     >  1:14448/1‑139 (MQ=255)

                                              aTGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAAt                                                                              >  2:761162/1‑139 (MQ=255)

                                                                              aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                                             <  1:593450/76‑1 (MQ=255)

                                                                              aTCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGAc                                                                                                             >  2:593450/1‑76 (MQ=255)

                                                                                      gTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTAc                                      <  1:138960/139‑1 (MQ=255)

                                                                                      gTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTAc                                      <  1:737081/139‑1 (MQ=255)

                                                                                      gTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTAc                                      <  1:604706/139‑1 (MQ=255)

                                                                                       ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                                            >  2:561028/1‑132 (MQ=255)

                                                                                       ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAg                                            <  1:561028/132‑1 (MQ=255)

                                                                                       ttGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACg                                     >  1:396274/1‑139 (MQ=255)

                                                                                       tagCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACg                                     >  1:59877/3‑139 (MQ=255)

                                                                                        tGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg                                    >  1:62013/1‑139 (MQ=255)

                                                                                        tGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg                                    <  2:738500/139‑1 (MQ=255)

                                                                                        tGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg                                    <  2:553314/139‑1 (MQ=255)

                                                                                        agCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACgg                                    <  2:59877/138‑1 (MQ=255)

                                                                                         gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                          >  2:13157/1‑52 (MQ=255)

                                                                                         gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCAc                                                                                                                          <  1:13157/52‑1 (MQ=255)

                                                                                         gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                   >  2:277462/1‑139 (MQ=255)

                                                                                         gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                   <  2:636904/139‑1 (MQ=255)

                                                                                         gCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                   >  1:636904/1‑139 (MQ=255)

                                                                                           tGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGac                                 <  2:213298/139‑1 (MQ=255)

                                                                                                                     gACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                   >  2:218642/1‑111 (MQ=255)

                                                                                                                     gACGTGTAATAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACggg                                   <  1:218642/111‑1 (MQ=255)

                                                                                                                          gtaataGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAgcgc  >  1:168075/1‑139 (MQ=255)

CTGCCGATGATGTACCTGGCGCTGTCCTACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTGCTGGAAGATCCGACGCGTCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGC  >  NC_000913/762614‑762874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A