Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,275,815	T→C	E5E (GAA→GAG)	narL ←	response regulator in two‑component regulatory system with NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,275,815	0	T	C	100.0%	229.9 / NA	63	E5E (GAA→GAG)	narL	response regulator in two‑component regulatory system with NarX
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/31); total (32/31)

GAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCG  >  NC_000913/1275679‑1275950

gagaCTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTc                                                                                                                                       <  1:878532/139‑1 (MQ=255)

  gaCTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCt                                                                                                                                     <  1:698458/139‑1 (MQ=255)

    cTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGa                                                                                                                                   <  1:567888/139‑1 (MQ=255)

     tCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                  >  1:1397504/1‑139 (MQ=255)

       cGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTa                                                                                                                                >  1:203132/1‑139 (MQ=255)

        gCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTAc                                                                                                                               >  1:21326/1‑139 (MQ=255)

          cAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTc                                                                                                                             <  2:203132/139‑1 (MQ=255)

           aGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCa                                                                                                                            <  2:1397504/139‑1 (MQ=255)

                  tACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  1:747904/139‑1 (MQ=255)

                  tACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATACCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  1:354366/139‑1 (MQ=255)

                       tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       >  2:1408802/1‑132 (MQ=255)

                       tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  1:1408802/132‑1 (MQ=255)

                        gTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTc                                                                                                                                       >  1:247805/1‑115 (MQ=255)

                        gTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                  >  2:975003/1‑120 (MQ=255)

                        gTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                  <  1:975003/120‑1 (MQ=255)

                         ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  2:1279811/1‑139 (MQ=255)

                         ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  2:1589887/1‑139 (MQ=255)

                         ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  2:817289/1‑139 (MQ=255)

                                   cTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  2:1589538/120‑1 (MQ=255)

                                   cTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       >  1:1589538/1‑120 (MQ=255)

                                        ttCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGa                                                                                                                                   <  2:1205110/103‑1 (MQ=255)

                                        ttCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGa                                                                                                                                   >  1:1205110/1‑103 (MQ=255)

                                        ttCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGt                                                                                               >  1:901290/1‑139 (MQ=255)

                                                      tGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATaa                                                                                 <  1:1279811/139‑1 (MQ=255)

                                                           tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTa                                                                                                                      <  2:561804/97‑1 (MQ=255)

                                                           tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTa                                                                                                                      >  1:561804/1‑97 (MQ=255)

                                                           tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTg                                                                            >  1:8926/1‑139 (MQ=255)

                                                                tGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGaccac                                                                       >  2:1043398/1‑139 (MQ=255)

                                                                     tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCgg                                                                                       >  2:1171308/1‑118 (MQ=255)

                                                                     tACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCgg                                                                                       <  1:1171308/118‑1 (MQ=255)

                                                                       cTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCg                                                                >  1:1301390/1‑139 (MQ=255)

                                                                           tAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGc                                                            <  1:1043398/139‑1 (MQ=255)

                                                                              gCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCAc                                                         <  1:1589887/139‑1 (MQ=255)

                                                                               cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     >  2:185120/1‑78 (MQ=255)

                                                                               cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  1:185120/78‑1 (MQ=255)

                                                                                            gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                         <  2:375800/93‑1 (MQ=255)

                                                                                            gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                         >  1:375800/1‑93 (MQ=255)

                                                                                            gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGAcgg                                           >  1:1091222/1‑139 (MQ=255)

                                                                                             ttCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGAcggc                                          >  1:1582521/1‑139 (MQ=255)

                                                                                                    caTCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGAcgcg                                   >  2:401054/1‑139 (MQ=255)

                                                                                                         ggTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAAt                              >  2:464403/1‑139 (MQ=255)

                                                                                                          gTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                             <  2:1301390/139‑1 (MQ=255)

                                                                                                            gATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAtct                                                                                                                   <  1:1359531/51‑1 (MQ=255)

                                                                                                            gATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAtct                                                                                                                   >  2:1359531/1‑51 (MQ=255)

                                                                                                                  cAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGCTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTa                     <  2:1091222/139‑1 (MQ=255)

                                                                                                                   aaTCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTC‑GGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTaaa                   >  2:1000340/1‑139 (MQ=255)

                                                                                                                     tCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                         <  1:1016163/68‑1 (MQ=255)

                                                                                                                     tCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTc                                                                                         >  2:1016163/1‑68 (MQ=255)

                                                                                                                      cagcagGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACt                 <  1:464403/139‑1 (MQ=255)

                                                                                                                      cagcagGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACt                 <  2:1582521/139‑1 (MQ=255)

                                                                                                                         cagGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTg              >  1:1022015/1‑139 (MQ=255)

                                                                                                                          agGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgc             >  1:417955/1‑139 (MQ=255)

                                                                                                                            gATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                             >  1:139182/1‑121 (MQ=255)

                                                                                                                            gATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                             <  2:139182/121‑1 (MQ=255)

                                                                                                                             aTAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa          <  2:8926/139‑1 (MQ=255)

                                                                                                                                 tagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGAt      >  2:733230/1‑139 (MQ=255)

                                                                                                                                   gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                             <  1:461942/66‑1 (MQ=255)

                                                                                                                                   gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                             >  2:461942/1‑66 (MQ=255)

                                                                                                                                   gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCg                            <  2:1345808/115‑1 (MQ=255)

                                                                                                                                   gCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCg                            >  1:1345808/1‑115 (MQ=255)

                                                                                                                                     cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  2:1022015/139‑1 (MQ=255)

                                                                                                                                     cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  1:1258804/139‑1 (MQ=255)

                                                                                                                                     cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  1:733230/139‑1 (MQ=255)

GAGACTCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCG  >  NC_000913/1275679‑1275950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A