Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,275,815	T→C	E5E (GAA→GAG)	narL ←	response regulator in two‑component regulatory system with NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,275,815	0	T	C	100.0%	159.4 / NA	45	E5E (GAA→GAG)	narL	response regulator in two‑component regulatory system with NarX
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/23); total (22/23)

TCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCG  >  NC_000913/1275684‑1275950

tCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                  <  2:149363/139‑1 (MQ=255)

tCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGAt                                                                                                                                  >  2:539838/1‑139 (MQ=255)

    ccAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACt                                                                                                                              <  2:999001/139‑1 (MQ=255)

    ccAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACt                                                                                                                              >  1:177058/1‑139 (MQ=255)

        ttCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATCAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATg                                                                                                                          >  2:699200/1‑139 (MQ=255)

        ttCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATg                                                                                                                          >  2:1068273/1‑139 (MQ=255)

           aaTACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  2:174580/139‑1 (MQ=255)

           aaTACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  2:177058/139‑1 (MQ=255)

           aaTACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGt                                                                                                                       <  1:608475/139‑1 (MQ=255)

            aTACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTa                                                                                                                      >  2:599375/1‑139 (MQ=255)

             tACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  2:991644/139‑1 (MQ=255)

               cccTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAtct                                                                                                                   <  1:489577/139‑1 (MQ=255)

                  tGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCt                                                                                                                >  2:451325/1‑139 (MQ=255)

                    ttCGCCCTTACTCGCTTCCCCAACCAGGGTGATATCTGGTGCCATACTGATAAGCTGTTTTATGCCAGTTTGCAGCATCGGTTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  2:560046/1‑139 (MQ=255)

                    ttCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTg                                                                                                              >  1:390845/1‑139 (MQ=255)

                                           ccACGGTGATATCTGGTGCCATACCGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCgg                                                                                       <  1:599375/139‑1 (MQ=255)

                                             aCGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGa                                                                                     <  1:451325/139‑1 (MQ=255)

                                                     atCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGt                                                                             <  2:550155/139‑1 (MQ=255)

                                                      tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCAt                                                                                                                           >  1:355773/1‑92 (MQ=255)

                                                      tCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCAt                                                                                                                           <  2:355773/92‑1 (MQ=255)

                                                        tGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGac                                                                          <  1:699200/139‑1 (MQ=255)

                                                          gTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGacca                                                                        >  2:862115/1‑139 (MQ=255)

                                                                     aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  1:889568/83‑1 (MQ=255)

                                                                     aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     >  2:889568/1‑83 (MQ=255)

                                                                     aTAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTg                                                             >  1:460723/1‑139 (MQ=255)

                                                                          cTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCAcc                                                        <  2:460723/139‑1 (MQ=255)

                                                                                       gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     >  1:1035583/1‑65 (MQ=255)

                                                                                       gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTAt                                                                                                                     <  2:1035583/65‑1 (MQ=255)

                                                                                       gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATa                                                                                  >  2:161911/1‑100 (MQ=255)

                                                                                       gTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATa                                                                                  <  1:161911/100‑1 (MQ=255)

                                                                                                 tCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGc                                 >  2:473733/1‑139 (MQ=255)

                                                                                                    ggTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAAt                              >  1:875416/1‑139 (MQ=255)

                                                                                                     gTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                             >  2:332989/1‑139 (MQ=255)

                                                                                                     gTGATCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATc                             <  2:390845/139‑1 (MQ=255)

                                                                                                         tCGTCAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCt                         >  1:361718/1‑139 (MQ=255)

                                                                                                             cAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGtt                                                                                              >  1:1014188/1‑66 (MQ=255)

                                                                                                             cAATCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGtt                                                                                              <  2:1014188/66‑1 (MQ=255)

                                                                                                              aaTCAGCAGGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTaa                    >  1:877447/1‑139 (MQ=255)

                                                                                                                 cagcagGATAGTAGCCGGCCCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACt                 <  1:560046/139‑1 (MQ=255)

                                                                                                                    cagGATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTg              >  1:957163/1‑139 (MQ=255)

                                                                                                                       gATAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTgcgc           >  1:807988/1‑139 (MQ=255)

                                                                                                                        aTAGTAGCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCa          <  1:473733/139‑1 (MQ=255)

                                                                                                                            tagCCGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGAt      <  2:875416/139‑1 (MQ=255)

                                                                                                                                cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  1:862115/139‑1 (MQ=255)

                                                                                                                                cGGCTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATcgcg  <  1:1068273/139‑1 (MQ=255)

TCCGCCAGTTCAATACCCTGTTCGCCATTACTCGCTTCGCCAACCACGGTGATATCTGGTGCCATACTGATAAGCTGTTTTACGCCAGTTCGCAGCATCGGGTGATCGTCAATCAGCAGGATAGTAGCCGGTTCCTGATTACTCATGGGTATCTCCTTGGACGTCTGTGAAAGTTTTTTCGGGAATAAAGGTGACCACCACTTCGGTGCCACCTGATTCACGACGGCGGACGCGGCAATCGCCTCGTAAACTTTGCGCACGATCGCG  >  NC_000913/1275684‑1275950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A