Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
780 |
68.3 |
3343050 |
93.5% |
3125751 |
102.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,606,561 |
A→G |
K154K (AAA→AAG) |
lsrF → |
putative autoinducer‑2 (AI‑2) aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,606,561 | 0 | A | G | 100.0%
| 128.4
/ NA
| 36 | K154K (AAA→AAG) | lsrF | putative autoinducer‑2 (AI‑2) aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/16); total (20/16) |
CGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCC > NC_000913/1606446‑1606681
|
cGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc > 2:429439/1‑139 (MQ=255)
cGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc > 1:362742/1‑139 (MQ=255)
gAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTgg < 1:389240/139‑1 (MQ=255)
cGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta > 2:872242/1‑104 (MQ=255)
cGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta < 1:872242/104‑1 (MQ=255)
aCGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGaa > 2:473745/1‑105 (MQ=255)
aCGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGaa < 1:473745/105‑1 (MQ=255)
gcgcCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGc > 2:1209724/1‑139 (MQ=255)
ttGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTggg > 1:1571092/1‑96 (MQ=255)
ttGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTggg < 2:1571092/96‑1 (MQ=255)
tGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAg < 1:1578890/69‑1 (MQ=255)
tGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAg > 2:1578890/1‑69 (MQ=255)
gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTc > 1:1004912/1‑66 (MQ=255)
gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTc < 2:1004912/66‑1 (MQ=255)
gcggcgCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGc > 2:546976/1‑139 (MQ=255)
gcggcgCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGc > 2:1207145/1‑139 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTgg > 2:1605730/1‑66 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTgg < 1:1605730/66‑1 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTg < 1:102354/110‑1 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTg > 2:102354/1‑110 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc > 2:527512/1‑139 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc > 1:1611536/1‑139 (MQ=255)
aaTATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAAt < 1:1209724/139‑1 (MQ=255)
aaTATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAAt < 1:531740/139‑1 (MQ=255)
aaTATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAAt > 2:531740/1‑139 (MQ=255)
tGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcgcc < 1:546976/139‑1 (MQ=255)
cATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCa < 2:204541/85‑1 (MQ=255)
cATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCa > 1:204541/1‑85 (MQ=255)
tCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTg < 1:953488/79‑1 (MQ=255)
tCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTg > 2:953488/1‑79 (MQ=255)
gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGa > 1:1458152/1‑68 (MQ=255)
gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGa < 2:1458152/68‑1 (MQ=255)
gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcgcc < 2:264571/130‑1 (MQ=255)
gTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcgcc > 1:264571/1‑130 (MQ=255)
gATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGc < 2:637689/60‑1 (MQ=255)
gATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGc > 1:637689/1‑60 (MQ=255)
|
CGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCC > NC_000913/1606446‑1606681
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
