Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,606,561 |
A→G |
K154K (AAA→AAG) |
lsrF → |
putative autoinducer‑2 (AI‑2) aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,606,561 | 0 | A | G | 100.0%
| 96.2
/ NA
| 27 | K154K (AAA→AAG) | lsrF | putative autoinducer‑2 (AI‑2) aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/14); total (13/14) |
CGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCG > NC_000913/1606431‑1606679
|
cGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTGTCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtattat > 1:648173/1‑139 (MQ=255)
cGGAATTAAGTAATGAAGACGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGTGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc < 1:626559/139‑1 (MQ=255)
ttAAGTAATGAAGCCGTGGCGTTGTCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGaa < 2:648173/139‑1 (MQ=255)
gTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGaa < 2:497491/139‑1 (MQ=255)
aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta > 1:204555/1‑108 (MQ=255)
aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAAtatta < 2:204555/108‑1 (MQ=255)
aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc > 2:551979/1‑124 (MQ=255)
aaGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGc < 1:551979/124‑1 (MQ=255)
gTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGAcc < 1:229926/139‑1 (MQ=255)
cgcCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCa > 1:57708/1‑139 (MQ=255)
gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg < 1:769198/120‑1 (MQ=255)
gcgcGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg > 2:769198/1‑120 (MQ=255)
cgcgGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTgcgc < 2:90720/139‑1 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc > 2:288634/1‑139 (MQ=255)
gCAGCGAATATGAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGAc > 2:293818/1‑139 (MQ=255)
gAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGat > 1:628038/1‑93 (MQ=255)
gAACATCAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGat < 2:628038/93‑1 (MQ=255)
cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGt < 2:933096/47‑1 (MQ=255)
cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGt > 1:933096/1‑47 (MQ=255)
cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATc > 2:595783/1‑100 (MQ=255)
cAGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATc < 1:595783/100‑1 (MQ=255)
aGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTggg > 2:357294/1‑79 (MQ=255)
aGTCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTggg < 1:357294/79‑1 (MQ=255)
tCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg < 2:659/73‑1 (MQ=255)
tCGATCAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCg > 1:659/1‑73 (MQ=255)
cAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcg < 2:393642/122‑1 (MQ=255)
cAAGAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATcg > 1:393642/1‑122 (MQ=255)
|
CGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGGATGACGCCGTGCGCCTGAACAGTTGCGCGGTGGCGGCGCAGGTTTATATCGGCAGCGAATATGAACATCAGTCGATCAAAAATATTATTCAGCTGGTTGATGCCGGAATGAAAGTGGGAATGCCGACCATGGCCGTGACTGGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCG > NC_000913/1606431‑1606679
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
