Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,324,876	A→G	L97P (CTG→CCG)	phnC ←	phosphonate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,324,876	0	A	G	100.0%	152.6 / NA	43	L97P (CTG→CCG)	phnC	phosphonate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/19); total (24/19)

GCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCA  >  NC_000913/4324743‑4325008

gCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGTGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTg                                                                                                                                 <  1:866077/139‑1 (MQ=255)

    aCGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAtt                                                                                                                             >  2:1659940/1‑139 (MQ=255)

     cgcgGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            >  2:297031/1‑139 (MQ=255)

         ggTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTgg                                                                                                                        >  2:428614/1‑139 (MQ=255)

          gTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGa                                                                                                                       >  1:1478406/1‑139 (MQ=255)

           tCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGaa                                                                                                                      >  1:1571798/1‑139 (MQ=255)

                gcCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            >  1:1086328/1‑128 (MQ=255)

                gcCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            <  2:1086328/128‑1 (MQ=255)

                 ccTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                <  1:1659940/139‑1 (MQ=255)

                 ccTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                <  1:428614/139‑1 (MQ=255)

                            cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGATTTGTTGGAATATGTAGCCGGTATggg                                                                                                     >  1:69081/1‑139 (MQ=255)

                             gcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                <  1:199994/127‑1 (MQ=255)

                             gcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                >  2:199994/1‑127 (MQ=255)

                                                      gCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                               >  2:1151072/1‑87 (MQ=255)

                                                      gCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                               <  1:1151072/87‑1 (MQ=255)

                                                                gTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                <  1:965847/92‑1 (MQ=255)

                                                                gTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                >  2:965847/1‑92 (MQ=255)

                                                                 tGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATc                                                                                  >  1:1201031/1‑121 (MQ=255)

                                                                 tGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATc                                                                                  <  2:1201031/121‑1 (MQ=255)

                                                                 tGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTc                                                                >  2:1132389/1‑139 (MQ=255)

                                                                   cgcCAGAACGGCGTGCTCCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTcgc                                                              >  1:957586/1‑139 (MQ=255)

                                                                     ccAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            <  2:40522/75‑1 (MQ=255)

                                                                     ccAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            >  1:40522/1‑75 (MQ=255)

                                                                              cGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                               <  2:214970/63‑1 (MQ=255)

                                                                              cGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                               >  1:214970/1‑63 (MQ=255)

                                                                                        aGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCcagca                                         <  2:1571798/139‑1 (MQ=255)

                                                                                           gcgcCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCt                                      <  1:1132389/139‑1 (MQ=255)

                                                                                                        cGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            >  1:949894/1‑40 (MQ=255)

                                                                                                        cGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                            <  2:949894/40‑1 (MQ=255)

                                                                                                           tctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGAc                      >  1:866099/1‑139 (MQ=255)

                                                                                                            ctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGata                                 >  2:1159429/1‑127 (MQ=255)

                                                                                                            ctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGata                                 <  1:1159429/127‑1 (MQ=255)

                                                                                                            ctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACa                     <  2:1478406/139‑1 (MQ=255)

                                                                                                                   aCGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGa                                                                                      >  2:519686/1‑67 (MQ=255)

                                                                                                                   aCGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGa                                                                                      <  1:519686/67‑1 (MQ=255)

                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCgg                                                                                                >  1:220962/1‑53 (MQ=255)

                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCgg                                                                                                <  2:220962/53‑1 (MQ=255)

                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCcagcag                                        <  1:1058076/109‑1 (MQ=255)

                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCcagcag                                        >  2:1058076/1‑109 (MQ=255)

                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCagcagc                                       >  2:921418/1‑110 (MQ=255)

                                                                                                                       tCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCagcagc                                       <  1:921418/110‑1 (MQ=255)

                                                                                                                           gCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTa      >  2:277330/1‑139 (MQ=255)

                                                                                                                               ttCACCGGGTTGAATTGTTGGAATATGTAACCAGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCATCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCa  >  2:1115519/1‑139 (MQ=255)

GCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCA  >  NC_000913/4324743‑4325008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A