Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,324,876	A→G	L97P (CTG→CCG)	phnC ←	phosphonate ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,324,876	0	A	G	100.0%	134.1 / NA	38	L97P (CTG→CCG)	phnC	phosphonate ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/21); total (17/21)

CATGCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCAAAC  >  NC_000913/4324740‑4325011

cATGCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCggg                                                                                                                                       >  2:1117723/1‑139 (MQ=255)

 aTGCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGt                                                                                                                                      <  2:583493/139‑1 (MQ=255)

     cAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGaa                                                                                                                                  <  1:1117723/139‑1 (MQ=255)

      aaCGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAt                                                                                                                                 <  2:676900/139‑1 (MQ=255)

       aCGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAtt                                                                                                                                >  1:750629/1‑139 (MQ=255)

        cgcgGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttg                                                                                                                               <  2:217682/139‑1 (MQ=255)

            ggTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTgg                                                                                                                           >  1:658931/1‑139 (MQ=255)

                 gcgcCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAatat                                                                                                                      >  1:1206891/1‑139 (MQ=255)

                               cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGTATATGTAGCCGGTATggg                                                                                                        >  2:477376/1‑139 (MQ=255)

                               cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATggg                                                                                                        >  1:1101086/1‑139 (MQ=255)

                               cgcTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATggg                                                                                                        >  2:201850/1‑139 (MQ=255)

                                  tGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTTAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGgcgc                                                                                                     <  1:591339/139‑1 (MQ=255)

                                    tttCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCgg                                                                                                   <  2:349907/139‑1 (MQ=255)

                                    tttCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCgg                                                                                                   <  2:329487/139‑1 (MQ=255)

                                               cGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGc                                                                                                                 <  1:407834/114‑1 (MQ=255)

                                               cGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGc                                                                                                                 >  2:407834/1‑114 (MQ=255)

                                                              aaCAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCgg                                                                                                   >  1:641996/1‑113 (MQ=255)

                                                              aaCAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCgg                                                                                                   <  2:641996/113‑1 (MQ=255)

                                                              aaCAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAggcg                                                                         >  2:780087/1‑139 (MQ=255)

                                                                       gcCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGTATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTcgcg                                                                <  1:477376/139‑1 (MQ=255)

                                                                                 cGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACtgtg                                                      >  2:992103/1‑139 (MQ=255)

                                                                                      tgcCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttgttg                                                                                                                            <  1:836443/64‑1 (MQ=255)

                                                                                      tgcCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAAttgttg                                                                                                                            >  2:836443/1‑64 (MQ=255)

                                                                                      tgcCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCGTTCGCGCTGGACTGTGCGGcc                                                 <  2:1101086/139‑1 (MQ=255)

                                                                                           aGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                   >  1:247086/1‑68 (MQ=255)

                                                                                           aGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTa                                                                                                                   <  2:247086/68‑1 (MQ=255)

                                                                                                  cAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGat                                     >  2:1234449/1‑139 (MQ=255)

                                                                                                      cAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATgg                                 <  2:114266/139‑1 (MQ=255)

                                                                                                              tctcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGAc                         <  1:992103/139‑1 (MQ=255)

                                                                                                                tcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCcag                                              >  2:248684/1‑116 (MQ=255)

                                                                                                                tcCAGTACGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCcag                                              <  1:248684/116‑1 (MQ=255)

                                                                                                                      aCGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGa                                                                                         >  1:397790/1‑67 (MQ=255)

                                                                                                                      aCGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGa                                                                                         <  2:397790/67‑1 (MQ=255)

                                                                                                                      aCGCTCAGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTAt                 <  2:1206891/139‑1 (MQ=255)

                                                                                                                            aGGCGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCgg           >  2:1283020/1‑139 (MQ=255)

                                                                                                                               cGGTTCACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTaa        <  1:201850/139‑1 (MQ=255)

                                                                                                                                    cACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACtgtg                                                      <  2:1084378/88‑1 (MQ=255)

                                                                                                                                    cACCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACtgtg                                                      >  1:1084378/1‑88 (MQ=255)

                                                                                                                                     aCCGGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGGCAGGCGGCCTTCGCGCTGGACTTTGCGGCCCAGCAGCTCGATATGGCTACCGGCAGATTTATCGCCGGTAATCAAAc  <  1:18515/139‑1 (MQ=255)

CATGCCAACGCGGGTCAGCGCCTGTAACGCGCGCTGTTTCTGCTCGCCGGTGAACCAGCTAAAACAGGTGCGCCAGAACGGCGTGCTGCCGAGCGCGCCAATCAGCACGTTCTCCAGTACGCTCAGGCGGTTCACCAGGTTGAATTGTTGGAATATGTAGCCGGTATGGGCGCGGCTTTTGCGGATATCGCGGGCCAGGCGGCCTTCGCGCTGGACTGTGCGGCCCAGCAGCTCGATATGGCTACCGACAGATTTATCGCCGGTAATCAAAC  >  NC_000913/4324740‑4325011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A