Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,247,620	C→T	A279A (GCC→GCT)	malK →	maltose ABC transportor ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,247,620	0	C	T	100.0%	77.2 / NA	25	A279A (GCC→GCT)	malK	maltose ABC transportor ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/13); total (12/13)

GGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCCAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTC  >  NC_000913/4247486‑4247753

ggTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAata                                                                                                                                   <  2:358412/139‑1 (MQ=255)

   tCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGt                                                                                                                                >  2:28679/1‑139 (MQ=255)

        aaaGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTg                                                                                                                           <  1:1001897/139‑1 (MQ=255)

              gAACTTCCTGCCGGTAAAAGGGACTGCCACTGCAATCGAGCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGGCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTAtt                                                                                                                     <  2:206671/139‑1 (MQ=255)

                  ttCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGcc                                                                                                                 <  2:496380/139‑1 (MQ=255)

                   tCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGccc                                                                                                                <  1:4300/139‑1 (MQ=255)

                                            cGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCgg                                                                                                              >  1:268397/1‑116 (MQ=255)

                                            cGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCgg                                                                                                              <  2:268397/116‑1 (MQ=255)

                                                               gTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAgg                                                                    >  1:524995/1‑139 (MQ=255)

                                                               gTGGAGCTGCCGATACCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAgg                                                                    >  2:400008/1‑139 (MQ=255)

                                                                   aGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGa                                                                >  1:923249/1‑139 (MQ=255)

                                                                         cGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTAc                                                                                                     <  2:938953/96‑1 (MQ=255)

                                                                         cGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTAc                                                                                                     >  1:938953/1‑96 (MQ=255)

                                                                                  aTCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGa                                                 >  2:365868/1‑139 (MQ=255)

                                                                                             gTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCt                                                                                                                            >  1:923383/1‑53 (MQ=255)

                                                                                             gTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCt                                                                                                                            <  2:923383/53‑1 (MQ=255)

                                                                                                     gCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGa                                                                      >  2:1791/1‑99 (MQ=255)

                                                                                                     gCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGa                                                                      <  1:1791/99‑1 (MQ=255)

                                                                                                       cAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCa                            <  1:400008/139‑1 (MQ=255)

                                                                                                             aaaGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCa                      <  1:365868/139‑1 (MQ=255)

                                                                                                                  cGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATcc                 <  2:923249/139‑1 (MQ=255)

                                                                                                                   gTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCa                >  2:422208/1‑139 (MQ=255)

                                                                                                                    tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                   <  1:779256/55‑1 (MQ=255)

                                                                                                                    tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                   >  2:779256/1‑55 (MQ=255)

                                                                                                                                 ggAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTc  >  1:239682/1‑139 (MQ=255)

GGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCCAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTC  >  NC_000913/4247486‑4247753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A