Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,254,003 |
C→T |
intergenic (+115/+40) |
ubiA → / ← plsB |
p‑hydroxybenzoate octaprenyltransferase/glycerol‑3‑phosphate O‑acyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,254,003 | 0 | C | T | 97.6%
| 124.3
/ ‑4.9
| 41 | intergenic (+115/+40) | ubiA/plsB | p‑hydroxybenzoate octaprenyltransferase/glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (19/21); total (20/21) |
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGT > NC_000913/4253885‑4254139
|
cTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTcgccgc < 2:668803/139‑1 (MQ=255)
tAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCaa < 2:1008382/139‑1 (MQ=255)
catcCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGTAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCt < 1:441531/139‑1 (MQ=255)
tcttctGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCg > 2:463041/1‑139 (MQ=255)
tcttctGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCg > 2:146539/1‑139 (MQ=255)
tGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTc < 1:146539/139‑1 (MQ=255)
ggggcTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACg < 2:996248/136‑1 (MQ=255)
ggcgCTTCTCAGGCCTACACAACACGTCGCATTTTATTGAATTTGCAGATTATGGAAGGCTGGATAAgg < 1:717978/69‑1 (MQ=255)
ggcgCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAgg > 2:717978/1‑69 (MQ=255)
ggcgCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCCTCGCCCTGCGTCGCACTCTCAATCGTCAAACg < 1:536369/139‑1 (MQ=255)
gcgcTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGc < 2:413448/139‑1 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCa < 1:319859/72‑1 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCa > 2:319859/1‑72 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATtct > 1:753595/1‑77 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATtct < 2:753595/77‑1 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACg > 2:49792/1‑122 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACg < 1:49792/122‑1 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTg > 1:688873/1‑130 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTg < 2:688873/130‑1 (MQ=255)
cacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCa > 2:265451/1‑139 (MQ=255)
acacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTc < 1:323577/139‑1 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCg < 1:712709/64‑1 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCg > 2:712709/1‑64 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCt > 1:1009399/1‑92 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCt < 2:1009399/92‑1 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTc > 1:984971/1‑97 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTc < 2:984971/97‑1 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTcc > 1:793432/1‑139 (MQ=255)
cacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTcc > 1:431721/1‑139 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGt > 1:30939/1‑91 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGt < 2:30939/91‑1 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCActct < 1:917540/99‑1 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCActct > 2:917540/1‑99 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt < 2:971776/132‑1 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACt > 1:971776/1‑132 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAg > 1:208121/1‑139 (MQ=255)
cGCAATTTATTGAATTTGCAGATTATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAg > 1:492473/1‑139 (MQ=255)
ttATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACg > 1:941051/1‑94 (MQ=255)
ttATGGAAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACg < 2:941051/94‑1 (MQ=255)
ggCTGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCgg < 2:793432/139‑1 (MQ=255)
gCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCCTCGTTTCTGCCGGt > 1:770392/1‑139 (MQ=255)
|
CTGATGATGTAAAAAAGCCGGATGATCATCCGGCTTTCTTCTGGGTTGCCTGATGCGCGGCGCTTCTCAGGCCTACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGT > NC_000913/4253885‑4254139
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
