Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,247,620	C→T	A279A (GCC→GCT)	malK →	maltose ABC transportor ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,247,620	0	C	T	100.0%	169.6 / NA	53	A279A (GCC→GCT)	malK	maltose ABC transportor ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/27); total (26/27)

CGGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCCAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGT  >  NC_000913/4247485‑4247755

cGGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGTCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAat                                                                                                                                      <  2:917727/139‑1 (MQ=255)

   ttCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATg                                                                                                                                   >  1:260616/1‑139 (MQ=255)

    tCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATg                                                                                                                                   >  1:467473/1‑138 (MQ=255)

    tCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATg                                                                                                                                   <  2:467473/138‑1 (MQ=255)

    tCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATAGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGt                                                                                                                                  >  1:1072364/1‑139 (MQ=255)

     cGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTc                                                                                                                                 <  1:404467/139‑1 (MQ=255)

      gCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCg                                                                                                                                <  1:1255642/139‑1 (MQ=255)

                      cTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCgg                                                                                                                >  2:205298/1‑139 (MQ=255)

                       tGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGa                                                                                                               >  1:178043/1‑139 (MQ=255)

                        gCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGaa                                                                                                              >  1:465638/1‑139 (MQ=255)

                             tAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCg                                                                                                                 <  2:517852/131‑1 (MQ=255)

                             tAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCg                                                                                                                 >  1:517852/1‑131 (MQ=255)

                                 aGTGACCGCCACCGCAATCGAGCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                     <  2:56109/139‑1 (MQ=255)

                                    gACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCg                                                                                                  >  2:467021/1‑139 (MQ=255)

                                       cGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGt                                                                                               >  2:1094398/1‑139 (MQ=255)

                                         ccACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGa                                                                                             <  1:863410/139‑1 (MQ=255)

                                           aCCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGata                                                                                           >  2:705809/1‑139 (MQ=255)

                                           aCCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGata                                                                                           <  1:961712/139‑1 (MQ=255)

                                                      tCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTc                                                                                >  2:590415/1‑139 (MQ=255)

                                                      tCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTc                                                                                <  1:1094398/139‑1 (MQ=255)

                                                           tGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCt                                                                           <  2:1235224/139‑1 (MQ=255)

                                                                gTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGccc                                                                                                                  <  2:1064022/95‑1 (MQ=255)

                                                                gTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGccc                                                                                                                  >  1:1064022/1‑95 (MQ=255)

                                                                gTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAgg                                                                      >  2:526312/1‑139 (MQ=255)

                                                                gTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAgg                                                                      >  2:1041606/1‑139 (MQ=255)

                                                                   gAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGa                                                                                                               >  2:346719/1‑95 (MQ=255)

                                                                   gAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGa                                                                                                               <  1:346719/95‑1 (MQ=255)

                                                                   gAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTg                                                                   <  2:1072364/139‑1 (MQ=255)

                                                                    aGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGTAGCTAATATGTCGCTGGGTATTCGccc                                                                                                                  <  2:1201620/91‑1 (MQ=255)

                                                                    aGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGccc                                                                                                                  >  1:1201620/1‑91 (MQ=255)

                                                                    aGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGa                                                                  >  2:590289/1‑139 (MQ=255)

                                                                     gCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGcc                                                                                                                   >  1:358531/1‑89 (MQ=255)

                                                                     gCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGcc                                                                                                                   <  2:358531/89‑1 (MQ=255)

                                                                                           cAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCg                                           <  1:526312/139‑1 (MQ=255)

                                                                                            aaGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCgg                                          <  1:467021/139‑1 (MQ=255)

                                                                                                    cTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGaaa                                  <  2:465638/139‑1 (MQ=255)

                                                                                                      gCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACt                                >  2:195444/1‑139 (MQ=255)

                                                                                                       ccAGTTGAAAGGCGTGATGTCCCGGTTGGAGTTAATATGTCTCTGGGTATTCGCCCGGAACATCTACTGCCGATTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTc                               <  2:1224474/139‑1 (MQ=255)

                                                                                                       ccAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTc                               <  1:1041606/139‑1 (MQ=255)

                                                                                                        cAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCt                                                                           <  2:214209/94‑1 (MQ=255)

                                                                                                        cAGTTGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCt                                                                           >  1:214209/1‑94 (MQ=255)

                                                                                                           ttGAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGGCGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAAt                           <  2:260616/139‑1 (MQ=255)

                                                                                                             gAAAGCCGTGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATcc                         <  1:590289/139‑1 (MQ=255)

                                                                                                                     tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCa                  >  1:370524/1‑138 (MQ=255)

                                                                                                                     tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCa                  <  2:370524/138‑1 (MQ=255)

                                                                                                                     tGATGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAg                 <  1:205298/139‑1 (MQ=255)

                                                                                                                        tGTCCAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATc              >  1:1158523/1‑139 (MQ=255)

                                                                                                                            cAGGTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCtt          >  1:1061911/1‑139 (MQ=255)

                                                                                                                               gTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                     <  2:1199067/45‑1 (MQ=255)

                                                                                                                               gTTGGAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTg                                                                                                     >  1:1199067/1‑45 (MQ=255)

                                                                                                                                  ggAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTc                               <  2:267638/112‑1 (MQ=255)

                                                                                                                                  ggAGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTc                               >  1:267638/1‑112 (MQ=255)

                                                                                                                                    aGCTAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGt  >  1:1268508/1‑139 (MQ=255)

CGGTTCGCCAAAGATGAACTTCCTGCCGGTAAAAGTGACCGCCACCGCAATCGATCAAGTGCAGGTGGAGCTGCCGATGCCAAATCGTCAGCAAGTCTGGCTGCCAGTTGAAAGCCGTGATGTCCAGGTTGGAGCCAATATGTCGCTGGGTATTCGCCCGGAACATCTACTGCCGAGTGATATCGCTGACGTCATCCTTGAGGGTGAAGTTCAGGTCGTCGAGCAACTCGGCAACGAAACTCAAATCCATATCCAGATCCCTTCCATTCGT  >  NC_000913/4247485‑4247755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A