Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,964	C→G	G190G (GGC→GGG)	ydiS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,964	0	C	G	100.0%	169.6 / NA	48	G190G (GGC→GGG)	ydiS	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (19/29); total (19/29)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCC  >  NC_000913/1781828‑1782090

aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  2:1325475/139‑1 (MQ=255)

aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  1:683069/139‑1 (MQ=255)

aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  1:1210733/139‑1 (MQ=255)

     ggggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCacac                                                                                                                         <  1:74221/139‑1 (MQ=255)

       ggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCa                                                                                                                       <  1:838288/139‑1 (MQ=255)

        gatgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAg                                                                                                                      <  1:666950/139‑1 (MQ=255)

        gatgatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGACGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAg                                                                                                                      <  2:965525/139‑1 (MQ=255)

           gatATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                   <  1:1276648/139‑1 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  2:244245/127‑1 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  1:705458/1‑127 (MQ=255)

              atTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGaa                                                                                                                    <  1:565781/135‑1 (MQ=255)

              atTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGaa                                                                                                                    >  2:565781/1‑135 (MQ=255)

                  tcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  2:693778/121‑1 (MQ=255)

                  tcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  1:693778/1‑121 (MQ=255)

                  tcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAg                                                                                                                      <  1:307669/129‑1 (MQ=255)

                  tcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAg                                                                                                                      >  2:307669/1‑129 (MQ=255)

                                   aTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                           <  2:415291/139‑1 (MQ=255)

                                     tCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACg                                                                                         >  1:263584/1‑139 (MQ=255)

                                             aTGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGa                                                                                 >  1:354759/1‑139 (MQ=255)

                                                   ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGa                                                                                                                     <  1:601053/97‑1 (MQ=255)

                                                   ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGa                                                                                                                     >  2:601053/1‑97 (MQ=255)

                                                   ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACCGATcgttgat                                                                                                        <  2:680342/110‑7 (MQ=255)

                                                   ttAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACCGATcgttgat                                                                                                        >  1:680342/1‑104 (MQ=255)

                                                           aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAg                                                                                                                      >  2:838367/1‑88 (MQ=255)

                                                           aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAg                                                                                                                      <  1:838367/88‑1 (MQ=255)

                                                           aTGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGc                                                                   >  1:513274/1‑139 (MQ=255)

                                                                     gctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCgg                                                         <  1:1181620/139‑1 (MQ=255)

                                                                      ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGc                                                                                      >  1:1005630/1‑109 (MQ=255)

                                                                      ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGt                                                        <  1:1258843/139‑1 (MQ=255)

                                                                      ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATAATACGGGc                                                                                      <  2:1005630/109‑1 (MQ=255)

                                                                       tcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGtt                                                       <  2:354759/139‑1 (MQ=255)

                                                                                   gTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGc                                                            >  1:881015/1‑122 (MQ=255)

                                                                                   gTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATAGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGc                                                            <  2:881015/122‑1 (MQ=255)

                                                                                          cTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGAt                                    <  2:263584/139‑1 (MQ=255)

                                                                                          cTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGAt                                    <  2:470858/139‑1 (MQ=255)

                                                                                              cGATCCGCATCATTACGCTGTTGGTGTTAAAGGGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGc                                >  2:1336265/1‑139 (MQ=255)

                                                                                               gATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCg                               <  1:1336265/139‑1 (MQ=255)

                                                                                                     catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                         <  2:938752/139‑1 (MQ=255)

                                                                                                     catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                         <  1:1197224/139‑1 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  2:549095/1‑47 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:104616/47‑1 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  2:104616/1‑47 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:549095/47‑1 (MQ=255)

                                                                                                                   tGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaac           >  2:1152228/1‑139 (MQ=255)

                                                                                                                    ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAtt                        <  2:1090094/125‑1 (MQ=255)

                                                                                                                    ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAtt                        >  1:1090094/1‑125 (MQ=255)

                                                                                                                          aaaGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGAtt    >  1:1085197/1‑139 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTcc  >  2:1533762/1‑139 (MQ=255)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCC  >  NC_000913/1781828‑1782090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A