Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,964	C→G	G190G (GGC→GGG)	ydiS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,964	0	C	G	100.0%	128.2 / NA	36	G190G (GGC→GGG)	ydiS	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (18/18); total (18/18)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATAT  >  NC_000913/1781828‑1782094

aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                  <  2:1245446/139‑1 (MQ=255)

  gCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTca                                                                                                                                <  2:1236764/139‑1 (MQ=255)

    tGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                  >  1:43972/1‑135 (MQ=255)

       ggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCa                                                                                                                           >  2:401383/1‑139 (MQ=255)

       ggATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCa                                                                                                                           <  1:540175/139‑1 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACa                                                                                                                      >  1:1222264/1‑139 (MQ=255)

                      aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGTATCACTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                            <  2:855003/139‑1 (MQ=255)

                      aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                            >  1:1222742/1‑139 (MQ=255)

                       gCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCCCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATc                                                                                                           >  1:991056/1‑139 (MQ=255)

                                gtgATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAata                                                                                                  >  2:765817/1‑139 (MQ=255)

                                  gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                       >  1:349885/1‑116 (MQ=255)

                                  gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                       <  2:349885/116‑1 (MQ=255)

                                              tGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                            >  1:218337/1‑115 (MQ=255)

                                              tGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                            <  2:218337/115‑1 (MQ=255)

                                                                                 tGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGa                                                 <  2:991056/139‑1 (MQ=255)

                                                                                 tGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGa                                                 <  2:1222742/139‑1 (MQ=255)

                                                                                   gTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                                  >  1:85772/1‑56 (MQ=255)

                                                                                       ccGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCacac                                                                                                                             >  2:140569/1‑57 (MQ=255)

                                                                                       ccGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCacac                                                                                                                             <  1:140569/57‑1 (MQ=255)

                                                                                              cGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACg                                                                                                              <  1:1223333/65‑1 (MQ=255)

                                                                                              cGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACg                                                                                                              >  2:1223333/1‑65 (MQ=255)

                                                                                                       tcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAttt                           <  1:401383/139‑1 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                       <  1:790094/36‑1 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAAc                                                                                                                       >  2:790094/1‑36 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                            <  1:692281/47‑1 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                            >  2:692281/1‑47 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa                >  1:756343/1‑139 (MQ=255)

                                                                                                                    ggTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaaca              >  2:246679/1‑139 (MQ=255)

                                                                                                                       gtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAgg           <  1:246679/139‑1 (MQ=255)

                                                                                                                          aaaGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGAtt        <  1:765817/139‑1 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaac               <  1:14878/130‑1 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaac               >  2:14878/1‑130 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg            <  2:993968/133‑1 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg            >  1:993968/1‑133 (MQ=255)

                                                                                                                                gTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCatat  >  1:174934/1‑139 (MQ=255)

                                                                                                                                     tGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTcccc                                                       <  2:830413/81‑1 (MQ=255)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCCATAT  >  NC_000913/1781828‑1782094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A