Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,964	C→G	G190G (GGC→GGG)	ydiS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,964	0	C	G	100.0%	182.1 / NA	51	G190G (GGC→GGG)	ydiS	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (26/25); total (26/25)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCC  >  NC_000913/1781828‑1782090

aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  1:1445363/139‑1 (MQ=255)

    tGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTcaca                                                                                                                          >  2:1149170/1‑139 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  1:701382/1‑127 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  2:701382/127‑1 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACa                                                                                                                  >  2:56264/1‑139 (MQ=255)

            atatTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACa                                                                                                                  >  1:533670/1‑139 (MQ=255)

                 ctcGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCa                                                                                                             >  1:842049/1‑139 (MQ=255)

                     aaGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGC‑TCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  2:1557917/139‑1 (MQ=255)

                      aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACCGATcgttgat                                                                                                        <  2:1569079/139‑7 (MQ=255)

                      aGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:1149170/139‑1 (MQ=255)

                       gCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATc                                                                                                       >  2:369639/1‑139 (MQ=255)

                                gtgATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  1:93073/1‑107 (MQ=255)

                                  gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  2:225643/105‑1 (MQ=255)

                                  gATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  1:225643/1‑105 (MQ=255)

                                   aTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTa                                                                                           <  2:533670/139‑1 (MQ=255)

                                     tCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACg                                                                                         >  2:274785/1‑139 (MQ=255)

                                       tGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACggg                                                                                       >  1:724845/1‑139 (MQ=255)

                                         gCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:1701001/120‑1 (MQ=255)

                                         gCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  2:1807605/1‑120 (MQ=255)

                                         gCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  2:1701001/1‑120 (MQ=255)

                                         gCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:1807605/120‑1 (MQ=255)

                                            gATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAgg                                                                                  <  2:10911/139‑1 (MQ=255)

                                             aTGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              <  1:849272/94‑1 (MQ=255)

                                             aTGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  2:849272/1‑94 (MQ=255)

                                             aTGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGa                                                                                 >  2:1091633/1‑139 (MQ=255)

                                              tGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCt                                                                                                                              >  2:162509/1‑93 (MQ=255)

                                                          gATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTgg                                                                    <  1:369639/139‑1 (MQ=255)

                                                                 ggCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTg                                                             >  2:811761/1‑139 (MQ=255)

                                                                 ggCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGATATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  2:610007/96‑1 (MQ=255)

                                                                 ggCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGATATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  1:610007/1‑96 (MQ=255)

                                                                      ctcgctGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGt                                                        >  2:1548691/1‑139 (MQ=255)

                                                                                         gCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGa                                     <  1:811761/139‑1 (MQ=255)

                                                                                             ccGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATggg                                 >  2:493525/1‑139 (MQ=255)

                                                                                                   cGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCggggg                           >  1:1712090/1‑139 (MQ=255)

                                                                                                    gCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                          <  2:842049/139‑1 (MQ=255)

                                                                                                    gCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                          <  1:493525/139‑1 (MQ=255)

                                                                                                     catcatTACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                         <  1:1548691/139‑1 (MQ=255)

                                                                                                           tACGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTctct                   <  2:1712090/139‑1 (MQ=255)

                                                                                                             cGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                         >  2:1507352/1‑131 (MQ=255)

                                                                                                             cGCTGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGAt                         <  1:1507352/131‑1 (MQ=255)

                                                                                                                tGTTGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATAcc              <  2:1802741/139‑1 (MQ=255)

                                                                                                                  ttGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaa            >  1:460212/1‑139 (MQ=255)

                                                                                                                   tGGTGTTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACcaac           <  2:460212/139‑1 (MQ=255)

                                                                                                                      tgtTAAAGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAg        <  2:724845/139‑1 (MQ=255)

                                                                                                                       gtTAAAGAGGTTATTGGGCTCACTCCAGAACAGATCAACGATCGCTTTAATATTTCGGGCGAGGAAGGTGCCGCCTGGCAGTTTTCCGGTTCCCCTTCTTACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAgg       <  1:1208820/139‑1 (MQ=255)

                                                                                                                          aaaGAGGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGAtt    >  2:692449/1‑139 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        >  2:356084/1‑37 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGAt                                                                                                        <  1:356084/37‑1 (MQ=255)

                                                                                                                            agagGTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCTGGGGATTTCTCTATACCAACAAGGATTcc  >  2:749065/1‑139 (MQ=255)

                                                                                                                                gTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                          <  2:1517536/111‑1 (MQ=255)

                                                                                                                                gTTATTGGGCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGa                          >  1:1517536/1‑111 (MQ=255)

AGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGATCGCTTTAATATTACGGGCGAGGAAGGTGCCGCCTGGCTGTTTGCCGGTTCCCCTTCTGACGGCCTGATGGGCGGGGGATTTCTCTATACCAACAAGGATTCC  >  NC_000913/1781828‑1782090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A