Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,809,787 |
T→C |
D136D (GAT→GAC) |
yniC → |
2‑deoxyglucose‑6‑P phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,809,787 | 0 | T | C | 100.0%
| 160.3
/ NA
| 45 | D136D (GAT→GAC) | yniC | 2‑deoxyglucose‑6‑P phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/21); total (24/21) |
ACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGATGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATCGCCTC > NC_000913/1809661‑1809921
|
aCCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGAGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcctcg > 2:887315/1‑139 (MQ=255)
tGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaa > 2:661203/1‑139 (MQ=255)
gcgcGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaaa > 1:252681/1‑139 (MQ=255)
gCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCa < 1:1459958/139‑1 (MQ=255)
aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATc < 2:1380354/137‑1 (MQ=255)
aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATc > 1:1380354/1‑137 (MQ=255)
aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg < 1:887315/139‑1 (MQ=255)
aTTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATAtctc > 2:1155433/1‑139 (MQ=255)
ggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTatat < 1:1454995/129‑1 (MQ=255)
ggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTatat > 2:1454995/1‑129 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaaa < 2:104168/85‑1 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaaa > 1:104168/1‑85 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCa > 2:611518/1‑98 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCa < 1:611518/98‑1 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACt > 2:279944/1‑138 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACt < 1:279944/138‑1 (MQ=255)
cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTg > 1:54619/1‑139 (MQ=255)
tGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGAc < 2:252681/139‑1 (MQ=255)
ggAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGc > 1:1206369/1‑139 (MQ=255)
gAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCg > 1:194485/1‑139 (MQ=255)
aaaaaGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGc < 1:1338330/87‑1 (MQ=255)
aaaaaGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGc > 2:1338330/1‑87 (MQ=255)
aaaaaGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGt > 2:1011625/1‑139 (MQ=255)
aGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTgcg < 2:353533/101‑1 (MQ=255)
aGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTgcg > 1:353533/1‑101 (MQ=255)
tGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcct > 1:523778/1‑42 (MQ=255)
tGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcct < 2:523778/42‑1 (MQ=255)
tGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGa < 2:194485/139‑1 (MQ=255)
tGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGa < 1:1155433/139‑1 (MQ=255)
ccATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGa < 2:54619/139‑1 (MQ=255)
aTGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGAtt > 1:297401/1‑139 (MQ=255)
aCTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCg > 1:388225/1‑117 (MQ=255)
aCTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCg < 2:388225/117‑1 (MQ=255)
cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc > 2:444492/1‑59 (MQ=255)
cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc < 1:444492/59‑1 (MQ=255)
cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg < 1:383232/60‑1 (MQ=255)
cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg > 2:383232/1‑60 (MQ=255)
cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCa < 1:400148/139‑1 (MQ=255)
cgcgACAGTTTCGACGACCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCa > 2:745723/1‑139 (MQ=255)
cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTggg < 1:1053091/87‑1 (MQ=255)
cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTggg > 2:1053091/1‑87 (MQ=255)
cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATc < 1:661203/139‑1 (MQ=255)
aGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTaa > 1:220899/1‑127 (MQ=255)
aGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTaa < 2:220899/127‑1 (MQ=255)
tCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATCGCctc > 2:529590/1‑139 (MQ=255)
|
ACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGATGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATCGCCTC > NC_000913/1809661‑1809921
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
