Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I1 R1
|
759 |
71.0 |
3673240 |
91.5% |
3361014 |
104.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,264,970 |
G→A |
*419* (TAG→TAA) |
hemA → |
glutamyl tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,264,970 | 0 | G | A | 100.0%
| 171.4
/ NA
| 53 | *419* (TAG→TAA) | hemA | glutamyl tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/29); total (24/29) |
CCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCG > NC_000913/1264837‑1265089
|
ccATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTa < 2:1012852/139‑1 (MQ=255)
gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCt > 1:1121021/1‑139 (MQ=255)
cATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTCACAGTACATCATTTTCTTTTTTTa < 2:1121021/139‑1 (MQ=255)
tGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 1:1460572/139‑1 (MQ=255)
tGGAAACGGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACTAACGCCTGCATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 1:1042387/139‑1 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:1067743‑M1/139‑5 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 1:766608‑M1/139‑5 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:121321‑M1/139‑5 (MQ=255)
aCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggagga > 1:975785‑M1/1‑131 (MQ=255)
ccGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaa > 1:899856‑M1/1‑130 (MQ=255)
ttGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataat < 1:713544‑M1/139‑14 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg < 2:428891/121‑1 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg > 1:428891/1‑121 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaata < 2:487306‑M1/136‑12 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaata > 1:487306‑M1/1‑125 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 2:1746432‑M1/1‑125 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 1:1746432‑M1/129‑5 (MQ=255)
tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTCgaggaggaataatg > 2:1129965‑M1/1‑125 (MQ=255)
gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg < 1:1129965‑M1/139‑16 (MQ=255)
gATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg < 2:848934‑M1/139‑16 (MQ=255)
gATCCACGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg < 1:1707206‑M1/139‑16 (MQ=255)
cAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagga < 1:1386682‑M1/104‑6 (MQ=255)
cAACAGGCCGCCCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagga > 2:1386682‑M1/1‑99 (MQ=255)
gccgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataag > 1:1385201‑M1/1‑93 (MQ=255)
cgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc > 1:1352734‑M1/1‑91 (MQ=255)
cgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc > 1:794716‑M1/1‑91 (MQ=255)
cgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:1749068‑M1/95‑5 (MQ=255)
cgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 1:231137‑M1/1‑91 (MQ=255)
cgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:231137‑M1/95‑5 (MQ=255)
cgccCGTGATGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 1:1749068‑M1/1‑91 (MQ=255)
atggggatAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACACTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcag < 2:975785‑M1/137‑57 (MQ=255)
aacgaacgCCTGGATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaat < 1:73459‑M1/118‑44 (MQ=255)
aacgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggcggaataatggaatatcaacactggttacgtgaagcaat > 2:73459‑M1/1‑75 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 1:1052559/69‑1 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa > 2:1052559/1‑69 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa < 2:358464‑M1/97‑24 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa > 1:358464‑M1/1‑74 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg < 1:1665168‑M1/89‑16 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgg > 2:1665168‑M1/1‑74 (MQ=255)
acgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 1:725519/1‑63 (MQ=255)
acgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 2:725519/63‑1 (MQ=255)
ccTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa > 2:1762522/1‑62 (MQ=255)
ccTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 1:1762522/62‑1 (MQ=255)
aaTATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc < 1:793708‑M1/111‑49 (MQ=255)
aaTATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc > 2:793708‑M1/1‑63 (MQ=255)
tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggagg > 2:1725941‑M1/1‑56 (MQ=255)
tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggagg < 1:1725941‑M1/63‑8 (MQ=255)
cgcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 1:724782/47‑1 (MQ=255)
cgcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 2:724782/1‑47 (MQ=255)
cgcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGt < 1:110259/53‑1 (MQ=255)
cgcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGt > 2:110259/1‑53 (MQ=255)
gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaa > 1:499223‑M1/1‑53 (MQ=255)
aCAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatc < 2:1385201‑M1/139‑90 (MQ=255)
|
CCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCG > NC_000913/1264837‑1265089
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
