Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
759 |
65.2 |
3386260 |
90.7% |
3071337 |
101.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,264,970 |
G→A |
*419* (TAG→TAA) |
hemA → |
glutamyl tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,264,970 | 0 | G | A | 100.0%
| 162.9
/ NA
| 50 | *419* (TAG→TAA) | hemA | glutamyl tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/25); total (25/25) |
TCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA > NC_000913/1264850‑1265103
|
tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCttt > 1:857083/1‑139 (MQ=255)
cATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 2:401030/139‑1 (MQ=255)
ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg < 1:25638/139‑1 (MQ=255)
tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 1:1390320/130‑1 (MQ=255)
tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 2:1390320/1‑130 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 1:1490783‑M1/139‑5 (MQ=255)
gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaata < 2:214836‑M1/139‑12 (MQ=255)
cTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGg > 2:1107796‑M1/1‑127 (MQ=255)
cTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGg < 1:1107796‑M1/128‑2 (MQ=255)
aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatat > 1:332551‑M1/1‑119 (MQ=255)
cgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa > 2:53583/1‑111 (MQ=255)
cgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 1:53583/111‑1 (MQ=255)
gcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaac < 2:332551‑M1/139‑25 (MQ=255)
ccAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaaca < 2:857083‑M1/139‑26 (MQ=255)
aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacact > 2:353270‑M1/1‑112 (MQ=255)
aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacact > 2:1104588‑M1/1‑112 (MQ=255)
aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacact > 1:1081519‑M1/1‑112 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 1:1532477/1‑86 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 2:1532477/86‑1 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataag > 1:761182‑M1/1‑93 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa > 2:710592‑M1/1‑93 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa < 1:710592‑M1/116‑24 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 1:903591‑M1/1‑93 (MQ=255)
gccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:903591‑M1/97‑5 (MQ=255)
cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAggg > 1:234653/1‑87 (MQ=255)
cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAggg < 2:234653/87‑1 (MQ=255)
cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaa > 1:831511‑M1/1‑89 (MQ=255)
cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaa > 2:788909‑M1/1‑89 (MQ=255)
ggggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggc < 2:831511‑M1/139‑59 (MQ=255)
gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaac > 2:838220‑M1/1‑78 (MQ=255)
gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaac < 1:838220‑M1/102‑25 (MQ=255)
gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa < 1:449986‑M1/101‑24 (MQ=255)
gATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaa > 2:449986‑M1/1‑78 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa > 1:355558/1‑69 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 2:355558/69‑1 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaa < 1:353270‑M1/139‑66 (MQ=255)
acgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaa < 2:761182‑M1/139‑66 (MQ=255)
cgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaa > 2:1412394‑M1/1‑73 (MQ=255)
gAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaat > 2:1435467‑M1/1‑64 (MQ=255)
gAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaat < 1:1435467‑M1/74‑11 (MQ=255)
tCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatg > 2:902544‑M1/1‑58 (MQ=255)
tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTc > 2:879679/1‑41 (MQ=255)
tGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTc < 1:879679/41‑1 (MQ=255)
cgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 2:277560‑M1/1‑52 (MQ=255)
cgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 1:277560‑M1/56‑5 (MQ=255)
tCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctgg < 2:1081519‑M1/139‑96 (MQ=255)
gCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagc < 1:788909‑M1/139‑100 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagcatgt > 1:1454640‑M1/1‑36 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcg > 1:974127‑M1/1‑36 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcg < 2:974127‑M1/99‑64 (MQ=255)
|
TCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA > NC_000913/1264850‑1265103
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
