Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
760 |
67.6 |
3323148 |
92.7% |
3080558 |
109.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,264,970 |
G→A |
*419* (TAG→TAA) |
hemA → |
glutamyl tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,264,970 | 0 | G | A | 100.0%
| 148.1
/ NA
| 46 | *419* (TAG→TAA) | hemA | glutamyl tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/28); total (18/28) |
GCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA > NC_000913/1264836‑1265103
|
gCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGt < 1:996963/139‑1 (MQ=255)
gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCt > 2:1647848/1‑139 (MQ=255)
gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCt > 1:1594878/1‑139 (MQ=255)
tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCttt < 1:1004016/139‑1 (MQ=255)
tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCttt > 1:816057/1‑139 (MQ=255)
cTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCGACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCtttt > 1:1118069/1‑139 (MQ=255)
ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCAGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCtttttt > 1:882825/1‑139 (MQ=255)
ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGAAGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCtttttt > 2:602746/1‑139 (MQ=255)
aTGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTAc < 2:882825/139‑1 (MQ=255)
ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTCCAg < 2:837310/139‑1 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 1:42536/1‑128 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 2:42536/128‑1 (MQ=255)
aCTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 1:807349‑M1/139‑5 (MQ=255)
tAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggag < 1:1078024‑M1/139‑7 (MQ=255)
aCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTc > 2:230052/1‑116 (MQ=255)
aCCGCGTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTc < 1:230052/116‑1 (MQ=255)
aTCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGAAGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatgga < 1:602746‑M1/139‑17 (MQ=255)
cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaata < 1:51111‑M1/139‑20 (MQ=255)
aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 1:797446‑M1/123‑5 (MQ=255)
aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 2:797446‑M1/1‑119 (MQ=255)
cgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa > 1:1413708/1‑111 (MQ=255)
cgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 2:1413708/111‑1 (MQ=255)
gcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaac < 1:1146022‑M1/139‑25 (MQ=255)
aCAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggagtaataatggaatatcaacactggttacgtgaagcaa < 2:1266097‑M1/139‑43 (MQ=255)
cgccCGTGACGGGGATAACGATCGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATGATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagcc < 2:966146‑M1/139‑49 (MQ=255)
cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaa > 1:1327203‑M1/1‑89 (MQ=255)
cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaa < 2:1327203‑M1/131‑43 (MQ=255)
gTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaactt < 2:236777‑M1/139‑54 (MQ=255)
aCGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaaattcag > 2:1111412‑M1/1‑83 (MQ=255)
acgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:963779‑M1/74‑5 (MQ=255)
acgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 1:963779‑M1/1‑70 (MQ=255)
cgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 2:152778/1‑62 (MQ=255)
cgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 1:152778/62‑1 (MQ=255)
cTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcg < 2:1118069‑M1/139‑74 (MQ=255)
cTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcg < 2:816057‑M1/139‑74 (MQ=255)
atatTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgt < 1:825021‑M1/139‑78 (MQ=255)
gcgcGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa > 1:229400/1‑48 (MQ=255)
gcgcGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTa < 2:229400/48‑1 (MQ=255)
gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc < 1:678358‑M1/74‑22 (MQ=255)
gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc > 2:678358‑M1/1‑53 (MQ=255)
gACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaa > 2:447557‑M1/1‑51 (MQ=255)
gACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaa < 1:447557‑M1/138‑88 (MQ=255)
gCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctg < 2:122423‑M1/139‑93 (MQ=255)
gCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagc < 2:1594878‑M1/139‑100 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagcatgt > 2:409893‑M1/1‑36 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagcatgt < 1:409893‑M1/139‑104 (MQ=255)
|
GCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA > NC_000913/1264836‑1265103
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
