Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,264,970 |
G→A |
*419* (TAG→TAA) |
hemA → |
glutamyl tRNA reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,264,970 | 0 | G | A | 100.0%
| 70.6
/ NA
| 23 | *419* (TAG→TAA) | hemA | glutamyl tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/10); total (13/10) |
TCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA > NC_000913/1264850‑1265103
|
tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCttt > 2:164034/1‑139 (MQ=255)
tGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGga > 1:219209‑M1/1‑137 (MQ=255)
aTGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatat > 1:464827‑M1/1‑119 (MQ=255)
aCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactg > 2:202848‑M1/1‑111 (MQ=255)
ggggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggc < 1:202848‑M1/139‑59 (MQ=255)
cgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg > 1:255142‑M1/1‑73 (MQ=255)
cgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgagg < 2:255142‑M1/77‑5 (MQ=255)
cgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataa > 2:636727‑M1/1‑69 (MQ=255)
cgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataa < 1:636727‑M1/81‑13 (MQ=255)
tCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggc > 2:97187‑M1/1‑58 (MQ=255)
tCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggc < 1:97187‑M1/116‑59 (MQ=255)
cgcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctga > 2:521330‑M1/1‑54 (MQ=255)
cgcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctga > 1:425053‑M1/1‑54 (MQ=255)
gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaata < 2:356841‑M1/72‑20 (MQ=255)
gcgACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaata > 1:356841‑M1/1‑53 (MQ=255)
aGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcct < 2:425053‑M1/139‑92 (MQ=255)
ccTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa > 1:617012/1‑41 (MQ=255)
ccTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACa < 2:617012/41‑1 (MQ=255)
tCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg < 1:757435/40‑1 (MQ=255)
tCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAg > 2:757435/1‑40 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatcaacactggttacgtgaagcaataagccaacttcaggcgagcgaaagcccgcggcgtgatgctgaaatcctgctggagcatgt < 1:521330‑M1/139‑104 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc < 1:502547‑M1/57‑22 (MQ=255)
gAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGgaggaggaataatggaatatc > 2:502547‑M1/1‑36 (MQ=255)
|
TCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGA > NC_000913/1264850‑1265103
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
