Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,276	G→A	392 (TAG→TAA)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,276	0	G	A	100.0%	167.4 / NA	52	392 (TAG→TAA)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/25); total (27/25)

TTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGC  >  NC_000913/696154‑696405

ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCg                                                                                                                   >  1:984464/1‑139 (MQ=255)

     tGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGc                                                                                                              >  2:90659/1‑139 (MQ=255)

     tGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGc                                                                                                              >  2:634696/1‑139 (MQ=255)

          gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                         <  2:984464/139‑1 (MQ=255)

                gCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGt                                                                                                   <  1:634696/139‑1 (MQ=255)

                            caccacTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTc                                                                                              >  1:1239632/1‑132 (MQ=255)

                            caccacTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTc                                                                                              <  2:1239632/132‑1 (MQ=255)

                                    cGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCa                                                                               <  2:1214290/139‑1 (MQ=255)

                                         tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAgg                                                                                                >  1:1050095/1‑117 (MQ=255)

                                         tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAgg                                                                                                <  2:1050095/117‑1 (MQ=255)

                                                       ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAg                                                                                                 >  2:364319/1‑102 (MQ=255)

                                                       ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAg                                                                                                 <  1:364319/102‑1 (MQ=255)

                                                       ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAg                                                                              <  2:748381/121‑1 (MQ=255)

                                                       ggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAg                                                                              >  1:748381/1‑121 (MQ=255)

                                                            aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCg                                                                                                                   >  2:101644/1‑79 (MQ=255)

                                                            aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCg                                                                                                                   <  1:101644/79‑1 (MQ=255)

                                                            aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGAt                                                       >  1:1441241/1‑139 (MQ=255)

                                                             tGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGaca                                                      >  2:1235636/1‑137 (MQ=255)

                                                              ggcggcGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                     <  2:602948/139‑1 (MQ=255)

                                                              ggcggcGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATaa                                                     <  1:1504511/139‑1 (MQ=255)

                                                                  gcggAGCGTGCTGGCGTGTTGAGACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACg                                                 >  1:414649/1‑139 (MQ=255)

                                                                  gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTc                                                                          <  2:27727/114‑1 (MQ=255)

                                                                  gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTc                                                                          >  1:27727/1‑114 (MQ=255)

                                                                                  tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                         >  1:748615/1‑131 (MQ=255)

                                                                                  tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                         <  2:748615/131‑1 (MQ=255)

                                                                                  tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGACAAGACGTGTCAACATCGCATTc                                 <  1:1235636/139‑1 (MQ=255)

                                                                                    ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                         <  1:470181/65‑1 (MQ=255)

                                                                                    ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                         >  2:470181/1‑65 (MQ=255)

                                                                                    ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATc                                                                     >  1:1161079/1‑101 (MQ=255)

                                                                                    ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATc                                                                     <  2:1161079/101‑1 (MQ=255)

                                                                                    ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                             >  1:873520/1‑109 (MQ=255)

                                                                                    ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                             <  2:873520/109‑1 (MQ=255)

                                                                                         aCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtg                          >  1:452224/1‑139 (MQ=255)

                                                                                            tCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaat                       <  1:90659/139‑1 (MQ=255)

                                                                                                   cTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCa                <  2:1441241/139‑1 (MQ=255)

                                                                                                           tGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACAtt                                                              >  1:1027141/1‑85 (MQ=255)

                                                                                                           tGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACAtt                                                              <  2:1027141/85‑1 (MQ=255)

                                                                                                             cGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCaaa      >  2:606403/1‑139 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                             <  2:1089343/81‑1 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                             >  1:1089343/1‑81 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCa        >  1:1351593/1‑134 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                               >  2:857118/1‑111 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                               <  1:857118/111‑1 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCa        <  2:1351593/134‑1 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCa        <  2:308515/134‑1 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAg   >  1:9049/1‑139 (MQ=255)

                                                                                                                tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGCCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAAAATCGCATTCGACATTGAATGAACGCAGAAAAGCa        >  1:308515/1‑134 (MQ=255)

                                                                                                                 aGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGc  >  2:336701/1‑139 (MQ=255)

                                                                                                                  gggTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAg   >  2:426419/1‑137 (MQ=255)

                                                                                                                  gggTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAg   <  1:426419/137‑1 (MQ=255)

                                                                                                                   ggTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtga                         <  2:1438924/114‑1 (MQ=255)

                                                                                                                   ggTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtga                         >  1:1438924/1‑114 (MQ=255)

TTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGC  >  NC_000913/696154‑696405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A