Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
696,276 |
G→A |
*392* (TAG→TAA) |
ubiF → |
2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 696,276 | 0 | G | A | 100.0%
| 29.9
/ NA
| 11 | *392* (TAG→TAA) | ubiF | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (3/8); total (3/8) |
GGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAGCCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAA > NC_000913/696163‑696387
|
ggATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGc < 2:568985/139‑1 (MQ=255)
gATTCAGCAATAATCTGCCACCCCTGGGTTTTA‑GCCGAATCCCGGGGTAATGGGCGCCGAGCCTGCCTGCGTGGTTAAACGTTAGGGCGTTG‑AATAATCGTTAAGGGTTGTACCCTAAAAAC‑TTGCCGGAATGCGTccc > 2:46156/1‑139 (MQ=255)
taatCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGt < 1:251169/139‑1 (MQ=255)
gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATc > 2:817620/1‑139 (MQ=255)
aaTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCg < 1:817620/139‑1 (MQ=255)
aaTCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCg < 2:718179/139‑1 (MQ=255)
aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGAt < 1:46156/139‑1 (MQ=255)
tGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa < 2:777744/139‑1 (MQ=255)
ttGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa > 2:605861/1‑139 (MQ=255)
cGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtga < 2:195517/139‑1 (MQ=255)
t‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa > 1:74698/1‑122 (MQ=255)
t‑AGGGTTGTAACCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatgaa < 2:74698/122‑1 (MQ=255)
|
GGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCA‑GGCGCTGAAATATGCGTT‑AGGGTTGTAGCCTTACAACATTGCCGG‑ATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAA > NC_000913/696163‑696387
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
