Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
696,276 |
G→A |
*392* (TAG→TAA) |
ubiF → |
2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 696,276 | 0 | G | A | 100.0%
| 74.6
/ NA
| 25 | *392* (TAG→TAA) | ubiF | 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/12); total (13/12) |
CGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGC > NC_000913/696141‑696405
|
cGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCt < 1:674771/139‑1 (MQ=255)
ttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGa < 2:994626/139‑1 (MQ=255)
tATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGAt < 2:682629/139‑1 (MQ=255)
aGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCg > 1:944441/1‑139 (MQ=255)
aataatCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAg < 2:870077/139‑1 (MQ=255)
tGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGATCGGAt > 1:893678/1‑139 (MQ=255)
cGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGATCGGATAAGACGCGGCa < 2:893678/139‑1 (MQ=255)
tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGTCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTacaac > 1:427582/1‑91 (MQ=255)
tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGTCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTacaac < 2:427582/91‑1 (MQ=255)
tATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTc > 2:682927/1‑139 (MQ=255)
aTGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCg > 1:1066154/1‑139 (MQ=255)
tctcGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGTGTTGTAACCTTACAACATTGCCGGATGCGTGTCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGaa < 1:682927/139‑2 (MQ=255)
aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTa < 1:855136/68‑1 (MQ=255)
aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTa > 2:855136/1‑68 (MQ=255)
gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGa > 1:506619/1‑137 (MQ=255)
gcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGa < 2:506619/137‑1 (MQ=255)
tGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCg > 1:2482/1‑139 (MQ=255)
tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAgg < 1:574986/76‑1 (MQ=255)
tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAgg > 2:574986/1‑76 (MQ=255)
ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa > 1:287900/1‑139 (MQ=255)
aatcGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACAt < 1:949942/136‑1 (MQ=255)
tGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCCACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCa > 1:727451/1‑139 (MQ=255)
ggTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGc > 2:60313/1‑137 (MQ=255)
ggTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGc < 1:60313/137‑1 (MQ=255)
gTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGAc > 2:157244/1‑85 (MQ=255)
|
CGGTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGC > NC_000913/696141‑696405
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
