Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,276	G→A	392 (TAG→TAA)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,276	0	G	A	100.0%	130.8 / NA	42	392 (TAG→TAA)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/23); total (19/23)

GTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCG  >  NC_000913/696143‑696408

gTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTa                                                                                                                                 <  1:599346/139‑1 (MQ=255)

  aTGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTaca                                                                                                                               <  2:456153/139‑1 (MQ=255)

           ttttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCg                                                                                                                      >  1:548975/1‑139 (MQ=255)

             ttATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGa                                                                                                                    <  2:548975/139‑1 (MQ=255)

               aTGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATg                                                                                                                  <  1:986217/139‑1 (MQ=255)

                    ggAGTCAGCAATAATCTGCCTCCTCTGCGTTTTCAGCTGAATCTCGGGGTAATGGCGTCTGAGCTTCCTCGCGTGTTTAACCGTCAGGCGCTTAAAAATGCCTTAGGGTTGAAACCTTACAACATGTCCGGATGCGTGc                                                                                                             <  2:1258229/139‑1 (MQ=255)

                     gATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGcc                                                                                                            >  2:1081048/1‑139 (MQ=255)

                               taatCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGt                                                                                                  <  2:296177/139‑1 (MQ=255)

                                  tCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCgg                                                                                               >  2:672242/1‑139 (MQ=255)

                                    tGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGAt                                                                                             >  1:230616/1‑139 (MQ=255)

                                      ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAgg                                                                                                   <  2:771950/131‑1 (MQ=255)

                                      ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAgg                                                                                                   >  1:771950/1‑131 (MQ=255)

                                      ccaccaCTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATaa                                                                                           >  2:805211/1‑139 (MQ=255)

                                         ccacTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGAc                                                                                        >  1:1463936/1‑139 (MQ=255)

                                           acTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGAcgc                                                                                      >  2:736739/1‑139 (MQ=255)

                                              gCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCCACCGTAGGTCGGATAAGACGCGGc                                                                                   >  2:893679/1‑139 (MQ=255)

                                                 ttttATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGc                                                                                >  2:382450/1‑139 (MQ=255)

                                                         gTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATc                                                                        <  1:736739/139‑1 (MQ=255)

                                                          tAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATcc                                                                       <  2:649415/139‑1 (MQ=255)

                                                            aTCTCGGGTTAATGGCGGCGGAGTGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGa                                                                     <  2:1087381/139‑1 (MQ=255)

                                                               tcGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACAt                                                                  <  1:1081048/139‑1 (MQ=255)

                                                                 gggTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTg                                                                <  2:230616/139‑1 (MQ=255)

                                                                       aTGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGAt                                                          >  1:1254101/1‑139 (MQ=255)

                                                                           cggcggAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGa                                                      >  1:990809/1‑139 (MQ=255)

                                                                                     tGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                            <  1:757552/139‑1 (MQ=255)

                                                                                            gtgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                                     <  1:382450/139‑1 (MQ=255)

                                                                                            gtgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCAtt                                     <  1:136785/139‑1 (MQ=255)

                                                                                             tgtTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTc                                    >  2:988756/1‑139 (MQ=255)

                                                                                               ttGAAACGTCAGGCGCTGGAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                                  >  2:921345/1‑139 (MQ=255)

                                                                                               ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                                  <  1:988756/139‑1 (MQ=255)

                                                                                               ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                                  <  1:492813/139‑1 (MQ=255)

                                                                                               ttGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGa                                  >  1:1522367/1‑139 (MQ=255)

                                                                                                 gAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACa                                <  2:1522367/139‑1 (MQ=255)

                                                                                                 gAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACa                                <  2:1254101/139‑1 (MQ=255)

                                                                                                    aCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGTATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                            <  1:1287480/124‑1 (MQ=255)

                                                                                                    aCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGCCGGATAAGACGCGGCAGCGTCTCATCCGACATTGAAGGATAAGACGTGTCAACa                                            >  2:1287480/1‑124 (MQ=255)

                                                                                                       tCAGTCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATttaat                          <  2:644602/139‑1 (MQ=255)

                                                                                                         aGGCGCTGAAATATGCGTTAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATtgaatga                        >  2:1255303/1‑139 (MQ=255)

                                                                                                                           tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                            <  2:1420516/101‑1 (MQ=255)

                                                                                                                           tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACa                                            >  1:1420516/1‑101 (MQ=255)

                                                                                                                           tAGGGTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAg      >  2:822258/1‑139 (MQ=255)

                                                                                                                               gTTGTAACCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg  <  2:1115337/139‑1 (MQ=255)

GTATGGATCTGTTTTATGCCGGATTCAGCAATAATCTGCCACCACTGCGTTTTATGCGTAATCTCGGGTTAATGGCGGCGGAGCGTGCTGGCGTGTTGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCG  >  NC_000913/696143‑696408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A