Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,950,559 |
(C)5→6 |
intergenic (‑37/‑273) |
aspS ← / → yecD |
aspartyl‑tRNA synthetase/isochorismatase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,950,554 | 1 | . | C | 97.8%
| 160.0
/ NA
| 46 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (25/20); minor base A (1/0); total (26/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTA > NC_000913/1950422‑1950682
|
aTCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccccc < 2:814652/139‑2 (MQ=255)
gCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCctgctg > 1:713633/1‑139 (MQ=255)
ccAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCa > 1:314347/1‑139 (MQ=255)
cAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAg > 1:682205/1‑139 (MQ=255)
acgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGc > 1:571790/1‑139 (MQ=255)
cgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCCTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCa > 1:504733/1‑139 (MQ=255)
gTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGc < 2:713633/139‑1 (MQ=255)
cccAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 2:314347/139‑1 (MQ=255)
ccACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa < 2:682205/139‑1 (MQ=255)
agagTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTcac > 2:456817/1‑139 (MQ=255)
tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 2:676758/1‑139 (MQ=255)
cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtg > 2:671155/1‑139 (MQ=255)
ccTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtgaa > 2:658822/1‑139 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCg < 1:426403/107‑1 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCg > 2:426403/1‑107 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGaaa > 1:424422/1‑139 (MQ=255)
ccccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 2:335016/1‑111 (MQ=255)
ccccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:335016/111‑1 (MQ=255)
ccACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAg < 1:671155/139‑1 (MQ=255)
cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 2:606788/112‑1 (MQ=255)
cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg > 1:606788/1‑112 (MQ=255)
gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTGTTATACTGGaa > 2:434158/1‑139 (MQ=255)
acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc < 2:424422/139‑1 (MQ=255)
tatTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa < 1:676758/139‑1 (MQ=255)
gTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg < 1:577724/131‑1 (MQ=255)
gTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg > 2:577724/1‑131 (MQ=255)
tACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGc > 1:745262/1‑68 (MQ=255)
tACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGc < 2:745262/68‑1 (MQ=255)
tACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAag > 2:458955/1‑139 (MQ=255)
aCGCATGAGATATCCCTTAACTTAGCTGACGGCGAATGACACACTGATGAGCAGGTAAGAAAGTCGCAGCGTTAGGTGTATGTCACAACAGAACGAAAAAAGGCGGCGATTATACTAGAAGTTGAGCCGCAACGTAaga > 2:36106/1‑139 (MQ=255)
gCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAagaga > 2:124605/1‑138 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACaa < 1:254881/84‑1 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACaa > 2:254881/1‑84 (MQ=255)
atatCCCTTAACTTAGCTGCCGGCGGATGCCCCCctgctg < 1:581912/40‑1 (MQ=255)
atatCCCTTAACTTAGCTGCCGGCGGATGCCCCCctgctg > 2:581912/1‑40 (MQ=255)
atatCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAAc > 2:741020/1‑80 (MQ=255)
atatCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAAc < 1:741020/80‑1 (MQ=255)
tCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCcgcg < 1:456817/139‑1 (MQ=255)
ccTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 2:655095/122‑1 (MQ=255)
ccTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa > 1:655095/1‑122 (MQ=255)
aaCTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGGTCTTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGaa > 1:530995/1‑139 (MQ=255)
cTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 2:398000/1‑52 (MQ=38)
cTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:398000/52‑1 (MQ=38)
aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCg > 2:522596/1‑43 (MQ=37)
aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCg < 1:522596/43‑1 (MQ=37)
ccGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:412194/139‑1 (MQ=255)
ccGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 1:458955/139‑1 (MQ=255)
|
ATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTA > NC_000913/1950422‑1950682
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
