Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,950,559	(C)_5→6	intergenic (‑37/‑273)	aspS ← / → yecD	aspartyl‑tRNA synthetase/isochorismatase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,950,554	1	.	C	100.0%	188.0 / NA	53	intergenic (‑32/‑278)	aspS/yecD	aspartyl‑tRNA synthetase/isochorismatase family protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (25/28); total (25/28)

ATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC  >  NC_000913/1950422‑1950683

aTCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑ccc                                                                                                                                <  1:687060/136‑1 (MQ=255)

aTCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑ccc                                                                                                                                >  2:687060/1‑136 (MQ=255)

    ggCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑cccccctgct                                                                                                                         <  2:25480/139‑6 (MQ=255)

          cAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAg                                                                                                                    >  2:635223/1‑139 (MQ=255)

          cAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAg                                                                                                                    <  2:649280/139‑1 (MQ=255)

                   gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAg                                                                                                           >  1:88685/1‑139 (MQ=255)

                        cgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCa                                                                                                      >  2:293838/1‑139 (MQ=255)

                                  aaCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg                                                                                            <  2:52201/139‑1 (MQ=255)

                                  aaCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg                                                                                            <  2:308669/139‑1 (MQ=255)

                                             gTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAAc                                                                                 >  2:335587/1‑139 (MQ=255)

                                              tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt                                                                                >  1:529401/1‑139 (MQ=255)

                                               cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg                                                                                               <  1:340070/123‑1 (MQ=255)

                                               cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg                                                                                               >  2:340070/1‑123 (MQ=255)

                                                 ccTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtgaa                                                                             >  2:168821/1‑139 (MQ=255)

                                                                 ggACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta                                                             <  1:777054/139‑1 (MQ=255)

                                                                       aCGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggcgg                                                                >  1:393206/1‑130 (MQ=255)

                                                                       aCGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggcgg                                                                <  2:393206/130‑1 (MQ=255)

                                                                            gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGc                                                                                                       >  2:138468/1‑86 (MQ=255)

                                                                            gCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGc                                                                                                       <  1:138468/86‑1 (MQ=255)

                                                                             cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGaaa                                                 <  1:335587/139‑1 (MQ=255)

                                                                                   acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc                                           <  1:293838/139‑1 (MQ=255)

                                                                                          tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt                                                                                                  >  2:874805/1‑77 (MQ=255)

                                                                                          tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt                                                                                                  <  1:874805/77‑1 (MQ=255)

                                                                                          tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc                                           >  2:192990/1‑132 (MQ=255)

                                                                                          tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc                                           <  1:192990/132‑1 (MQ=255)

                                                                                          tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCg                                    >  2:615619/1‑139 (MQ=255)

                                                                                            tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGAACCGTa                                  <  2:88685/139‑1 (MQ=255)

                                                                                              tACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa                                  >  2:653926/1‑137 (MQ=255)

                                                                                              tACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa                                  <  1:653926/137‑1 (MQ=255)

                                                                                               aCGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTc                                                                                                         >  2:431897/1‑65 (MQ=255)

                                                                                               aCGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTc                                                                                                         <  1:431897/65‑1 (MQ=255)

                                                                                                 gCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg                                                                                            <  2:587661/76‑1 (MQ=255)

                                                                                                 gCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg                                                                                            >  1:587661/1‑76 (MQ=255)

                                                                                                   aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt                           >  1:454996/1‑139 (MQ=255)

                                                                                                           tCCCTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCcgcg                   <  1:281670/139‑1 (MQ=255)

                                                                                                            cccTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACaa                                                                                  >  1:23924/1‑75 (MQ=255)

                                                                                                            cccTTAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACaa                                                                                  <  2:23924/75‑1 (MQ=255)

                                                                                                               ttAACTTAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTgg               >  1:472585/1‑139 (MQ=255)

                                                                                                                     tAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg                                                                                            <  1:191543/56‑1 (MQ=39)

                                                                                                                     tAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg                                                                                            >  2:191543/1‑56 (MQ=39)

                                                                                                                     tAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc                                           >  2:219459/1‑105 (MQ=255)

                                                                                                                     tAGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc                                           <  1:219459/105‑1 (MQ=255)

                                                                                                                      aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc                                                                  >  2:36629/1‑81 (MQ=255)

                                                                                                                      aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc                                                                  <  1:36629/81‑1 (MQ=255)

                                                                                                                      aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc                                                                  <  1:847433/81‑1 (MQ=255)

                                                                                                                      aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc                                                                  >  2:847433/1‑81 (MQ=255)

                                                                                                                      aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta                                                             <  1:468364/86‑1 (MQ=255)

                                                                                                                      aGCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta                                                             >  2:468364/1‑86 (MQ=255)

                                                                                                                       gCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta                                                             >  2:287067/1‑85 (MQ=255)

                                                                                                                       gCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCta                                                             <  1:287067/85‑1 (MQ=255)

                                                                                                                       gCTGCCGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTc       >  2:783969/1‑139 (MQ=255)

                                                                                                                           ccGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa   <  1:615619/139‑1 (MQ=255)

                                                                                                                           ccGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa   <  2:472585/139‑1 (MQ=255)

                                                                                                                           ccGGCGGATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa   <  1:635223/139‑1 (MQ=255)

                                                                                                                            cggcggATGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc  <  1:783969/139‑1 (MQ=255)

                                                                                                                            cggcggACGCCCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc  <  1:168821/139‑1 (MQ=255)

ATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑CCCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC  >  NC_000913/1950422‑1950683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A