Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,950,559 |
(C)5→6 |
intergenic (‑37/‑273) |
aspS ← / → yecD |
aspartyl‑tRNA synthetase/isochorismatase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,950,554 | 1 | . | C | 98.0%
| 172.9
/ ‑3.3
| 49 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (25/23); total (26/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 |
AAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑C‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950419‑1950683
|
aaGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑c‑cc < 2:10000/139‑1 (MQ=255)
cAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAg > 2:952063/1‑139 (MQ=255)
acgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGc < 2:736103/139‑1 (MQ=255)
tGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAAGTTAGCTGCCTGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:622770/139‑1 (MQ=255)
aaCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 1:952063/139‑1 (MQ=255)
cacacaGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTAt > 1:410315/1‑139 (MQ=255)
gTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 1:1745/1‑122 (MQ=255)
gTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:1745/122‑1 (MQ=255)
tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 2:28107/1‑139 (MQ=255)
tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 1:198829/1‑139 (MQ=255)
cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtg > 2:958505/1‑139 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCTACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa < 1:688998/94‑1 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCa > 2:688998/1‑94 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:430065/113‑1 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 1:430065/1‑113 (MQ=255)
tgcCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGaaa > 1:904568/1‑139 (MQ=255)
cACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCctgctg > 1:732186/1‑85 (MQ=255)
cACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCctgctg < 2:732186/85‑1 (MQ=255)
aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:28107/139‑1 (MQ=255)
aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 2:904568/139‑1 (MQ=255)
aCGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:874985/139‑1 (MQ=255)
cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 1:703654/1‑106 (MQ=255)
cGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 2:703654/106‑1 (MQ=255)
gTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggcgg < 2:198829/139‑1 (MQ=255)
gggACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCt > 2:327322/1‑139 (MQ=255)
aCGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATAc > 2:957971/1‑139 (MQ=255)
cGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTTCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAGTGAAAAAAGGAGGCTATTATACt > 1:361914/1‑139 (MQ=255)
tCCACAATCTTCTGTACGCATGAGATCTCCCTTAACTTAGCTGCCGGCGGATG‑CTCCCCTGATGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACGGAATGAAAAAAGGCGGCTTTTATACTGGAAATTc > 1:905051/1‑139 (MQ=255)
tCCACAATATTCTGTACGCATGAGATATCTCTTAACTTAGCTGCCAGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg > 1:583110/1‑90 (MQ=255)
tCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 2:583110/90‑1 (MQ=255)
acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGt > 2:513535/1‑95 (MQ=255)
acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGt < 1:513535/95‑1 (MQ=255)
tatTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa < 2:410315/139‑1 (MQ=255)
tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa > 1:274864/1‑85 (MQ=255)
tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTa < 2:274864/85‑1 (MQ=255)
tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 1:327322/139‑1 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTc < 2:713374/61‑1 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTc > 1:713374/1‑61 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt < 1:836862/68‑1 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGt > 2:836862/1‑68 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:564710/1‑139 (MQ=255)
gaTATCCCTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 1:564710/139‑1 (MQ=255)
ccTTAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCt > 1:446016/1‑139 (MQ=255)
tAACTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGa > 1:128436/1‑139 (MQ=255)
aaCTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa < 2:22811/113‑1 (MQ=255)
aaCTTAGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCa > 1:22811/1‑113 (MQ=255)
aGCTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 1:278137/1‑139 (MQ=255)
cTGCCGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCg > 2:243100/1‑139 (MQ=255)
ccGGCGGATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:278137/139‑1 (MQ=255)
cggcggATGCC‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc < 2:446016/139‑1 (MQ=255)
|
AAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑C‑CCCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAC > NC_000913/1950419‑1950683
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
