Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,950,559 |
(C)5→6 |
intergenic (‑37/‑273) |
aspS ← / → yecD |
aspartyl‑tRNA synthetase/isochorismatase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,950,554 | 1 | . | C | 97.7%
| 155.2
/ ‑3.5
| 44 | intergenic (‑32/‑278) | aspS/yecD | aspartyl‑tRNA synthetase/isochorismatase family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (22/21); total (22/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTACC > NC_000913/1950418‑1950684
|
gAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc > 1:511069/1‑139 (MQ=255)
aaGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑c < 2:1164255/139‑1 (MQ=255)
ggCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑cccctgct < 2:1176404/139‑6 (MQ=255)
tCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGAc > 2:787413/1‑139 (MQ=255)
cACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCAGCGGATG‑‑CCGCCCTGCTGCGCAGGTGAcc < 1:877103/139‑1 (MQ=255)
acgacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCa > 1:729704/1‑139 (MQ=255)
gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAg > 2:325578/1‑139 (MQ=255)
gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCC‑‑‑‑CTTAGCTGCCGGCGGATG‑‑cc‑cccc < 1:245784/116‑2 (MQ=255)
gacgacgGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCC‑‑‑‑CTTAGCTGCCGGCGGATG‑‑cc‑cccc > 2:245784/1‑115 (MQ=255)
aCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑cccctg < 2:48347/106‑4 (MQ=255)
aCCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑cc‑cccctg > 1:48347/1‑103 (MQ=255)
tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 1:1065170/1‑139 (MQ=255)
tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 2:922353/1‑139 (MQ=255)
tCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACt > 1:581325/1‑139 (MQ=255)
cACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACtg > 1:634229/1‑139 (MQ=255)
gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCGTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 2:927796/1‑133 (MQ=255)
gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 1:927796/133‑1 (MQ=255)
gACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCtat > 2:1013251/1‑139 (MQ=255)
cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGc > 2:858346/1‑88 (MQ=255)
cTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTACCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGc < 1:858346/88‑1 (MQ=255)
acaATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGc < 1:188157/139‑1 (MQ=255)
aaTATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg > 2:1074667/1‑139 (MQ=255)
tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc > 1:836205/1‑109 (MQ=255)
tCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAggc < 2:836205/109‑1 (MQ=255)
tGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 1:1013251/139‑1 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa < 1:406484/132‑1 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTa > 2:406484/1‑132 (MQ=255)
aTGAGATATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCt > 2:207901/1‑139 (MQ=255)
agaTATCCCTTAACTTAGCTGCCGGTGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGc > 1:3583/1‑139 (MQ=255)
gaTATCCCTTAACTTAGCTGCCGGTGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 2:3583/139‑1 (MQ=255)
gaTATCCCTTAACTTAGCTGCCGGCGGATGCCCC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGc < 2:267629/139‑1 (MQ=255)
gaTATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 2:88608/139‑1 (MQ=255)
gaTATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 2:511069/139‑1 (MQ=255)
gaTATCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGcc < 1:1074667/139‑1 (MQ=255)
atatCCCTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGccc > 2:467612/1‑139 (MQ=255)
cccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg > 2:247937/1‑116 (MQ=255)
cccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCg < 1:247937/116‑1 (MQ=255)
ccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg < 1:803809/61‑1 (MQ=255)
ccTTAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAg > 2:803809/1‑61 (MQ=255)
tAACTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGa < 1:325578/139‑1 (MQ=255)
aaCTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg > 1:924112/1‑60 (MQ=39)
aaCTTAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTg < 2:924112/60‑1 (MQ=39)
tAGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGcc > 2:124612/1‑139 (MQ=255)
aGCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCt > 2:793808/1‑139 (MQ=255)
gCTGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTc < 2:581325/139‑1 (MQ=255)
tGCCGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGt < 1:467612/139‑1 (MQ=255)
ccGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:729704/139‑1 (MQ=255)
ccGGCGGATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTa < 2:94832/139‑1 (MQ=255)
cggcggATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAc < 1:787413/139‑1 (MQ=255)
ggcggATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAcc < 1:922353/139‑1 (MQ=255)
ggcggATGC‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTAcc > 2:1180133/1‑139 (MQ=255)
|
GAAGATCAGGCTACCAAGATCACGACGACGGTTGACCCAACCACACAGAGTCACCTGCTGCCCCACGTGGGACAAACGGAGCTGTCCACAATATTCTGTACGCATGAGATATCCCTTAACTTAGCTGCCGGCGGATG‑‑CC‑CCCTGCTGCGCAGGTGACCAAGTCGCAGCGTTAGCTGTATGTCACAACTGAATGAAAAAAGGCGGCTATTATACTGGAAATTCTGCCGCACCGTAAGAGCCTGGCCCGCGCTGGAACGCCTCGTTACC > NC_000913/1950418‑1950684
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
