Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,333	T→C	E427E (GAA→GAG)	yhbX ←	putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,333	0	T	C	100.0%	206.2 / NA	57	E427E (GAA→GAG)	yhbX	putative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/26); total (31/26)

CCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAA  >  NC_000913/3320230‑3320466

ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                                    >  2:134696/1‑139 (MQ=255)

ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                                    >  2:596057/1‑139 (MQ=255)

ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                                    >  1:401116/1‑139 (MQ=255)

         cGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATc                                                                                           >  1:609084/1‑139 (MQ=255)

            ggCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCttt                                                                                        >  1:744812/1‑139 (MQ=255)

            ggCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCttt                                                                                        >  1:533563/1‑139 (MQ=255)

                      gATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCa                                                                              >  1:55088/1‑139 (MQ=255)

                       aTAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCaa                                                                             >  1:258526/1‑139 (MQ=255)

                           aCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGCCCGAGGCGCGGCGATCTTTTAATAATTCAAAAAc                                                                         >  2:413502/1‑139 (MQ=255)

                              tCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTg                                                                      >  2:365715/1‑139 (MQ=255)

                               cGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAGATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGa                                                                     >  2:592050/1‑139 (MQ=255)

                                gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGAc                                                                    <  1:365715/139‑1 (MQ=255)

                                 ggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGGcc                                                                   >  1:487642/1‑139 (MQ=255)

                                 ggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGAcc                                                                   >  1:760792/1‑139 (MQ=255)

                                    cATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAg                                                                <  1:592050/139‑1 (MQ=255)

                                      tGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCa                                                              <  2:744812/139‑1 (MQ=255)

                                            gCCTGCTGGCTAGCCTCCCTGACTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGGCCTAGCAAGCTAt                                                        <  2:487642/139‑1 (MQ=255)

                                            gCCTGCTGGCTAGCCGCCCTGACTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        <  1:596057/139‑1 (MQ=255)

                                             ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATc                                                       <  2:701322/139‑1 (MQ=255)

                                                 ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     <  2:7841/73‑1 (MQ=255)

                                                 ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     >  1:7841/1‑73 (MQ=255)

                                                 ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCTCGGCGTTCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        <  1:560658/134‑1 (MQ=255)

                                                 ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        >  2:560658/1‑134 (MQ=255)

                                                 ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                                   >  1:493008/1‑139 (MQ=255)

                                                   ggCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               <  1:263771/93‑1 (MQ=255)

                                                   ggCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               >  2:263771/1‑93 (MQ=255)

                                                   ggCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTa                                                 <  2:401116/139‑1 (MQ=255)

                                                   ggCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTa                                                 <  1:134696/139‑1 (MQ=255)

                                                   ggCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTa                                                 >  1:787157/1‑139 (MQ=255)

                                                     cTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTaat                                               <  2:787157/139‑1 (MQ=255)

                                                      tAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTaatg                                              >  2:3515/1‑139 (MQ=255)

                                                            cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAACCTAGCTGTGTAATGAATGGa                                        <  2:493008/139‑1 (MQ=255)

                                                            cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                        <  1:787602/139‑1 (MQ=255)

                                                            cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                        <  2:609084/139‑1 (MQ=255)

                                                             ccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAg                                       <  1:3515/139‑1 (MQ=255)

                                                              cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaa                                                                                     >  2:745925/1‑92 (MQ=255)

                                                              cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaa                                                                                     <  1:745925/92‑1 (MQ=255)

                                                                      aTGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAg                              >  2:101773/1‑139 (MQ=255)

                                                                       tGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGc                             >  2:157483/1‑139 (MQ=255)

                                                                           aaTAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGc                         >  2:788776/1‑139 (MQ=255)

                                                                                    tcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCa                            <  2:399069/127‑1 (MQ=255)

                                                                                    tcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCa                            >  1:399069/1‑127 (MQ=255)

                                                                                           cGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGc         <  2:533563/139‑1 (MQ=255)

                                                                                           cGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGc         <  1:495324/139‑1 (MQ=255)

                                                                                              aGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaata                                                                                   <  1:648230/62‑1 (MQ=255)

                                                                                              aGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaata                                                                                   >  2:648230/1‑62 (MQ=255)

                                                                                              aGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTaatga                                             >  2:275902/1‑100 (MQ=255)

                                                                                              aGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTaatga                                             <  1:275902/100‑1 (MQ=255)

                                                                                                ggTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               <  2:829792/48‑1 (MQ=255)

                                                                                                ggTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               >  1:829792/1‑48 (MQ=255)

                                                                                                  tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTa                                                         >  1:563089/1‑84 (MQ=255)

                                                                                                  tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTa                                                         <  2:563089/84‑1 (MQ=255)

                                                                                                  tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        >  1:299313/1‑85 (MQ=255)

                                                                                                  tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        <  2:299313/85‑1 (MQ=255)

                                                                                                  tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGaa  >  2:470035/1‑139 (MQ=255)

                                                                                                   cACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAg                                       >  1:509491/1‑101 (MQ=255)

                                                                                                   cACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAg                                       <  2:509491/101‑1 (MQ=255)

CCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAA  >  NC_000913/3320230‑3320466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A