Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,333	T→C	E427E (GAA→GAG)	yhbX ←	putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,333	0	T	C	100.0%	230.9 / NA	63	E427E (GAA→GAG)	yhbX	putative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/30); total (33/30)

ATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTG  >  NC_000913/3320199‑3320458

aTGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCa                                                                                                                           >  1:8340/1‑139 (MQ=255)

       tttCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTg                                                                                                                    <  2:1329/139‑1 (MQ=255)

          ccGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTg                                                                                                                    <  2:523081/136‑1 (MQ=255)

          ccGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTg                                                                                                                    >  1:523081/1‑136 (MQ=255)

              tgtgCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             <  1:793424/139‑1 (MQ=255)

                 gCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCaaaa                                                                                                          <  2:402687/139‑1 (MQ=255)

                     tCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGcc                                                                                                                       <  2:383433/122‑1 (MQ=255)

                     tCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGcc                                                                                                                       >  1:383433/1‑122 (MQ=255)

                     tCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACa                                                                                                      <  1:1152569/139‑1 (MQ=255)

                               ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAgg                                                                                                                         <  2:1300074/110‑1 (MQ=255)

                               ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAgg                                                                                                                         >  1:1300074/1‑110 (MQ=255)

                               ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                            >  1:248176/1‑139 (MQ=255)

                               ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                            >  2:655568/1‑139 (MQ=255)

                               ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                            >  2:305426/1‑139 (MQ=255)

                                  cAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTTGGCAAAATACATGACCGAGGcgcg                                                                                         >  1:480437/1‑139 (MQ=255)

                                    aGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                       <  2:480437/139‑1 (MQ=255)

                                         ggggCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCt                                                                                  >  1:871225/1‑139 (MQ=255)

                                                             tCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTg                                                              <  2:871225/139‑1 (MQ=255)

                                                               gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGAc                                                            >  1:194615/1‑139 (MQ=255)

                                                               gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGAc                                                            >  2:395286/1‑139 (MQ=255)

                                                               gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGAc                                                            >  2:167734/1‑139 (MQ=255)

                                                                        tatGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAAc                                                   <  1:167734/139‑1 (MQ=255)

                                                                           gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCa                                                      <  2:1241984/133‑1 (MQ=255)

                                                                           gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCa                                                      >  1:1241984/1‑133 (MQ=255)

                                                                           gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                <  2:118317/139‑1 (MQ=255)

                                                                           gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                <  2:8340/139‑1 (MQ=255)

                                                                             ctgctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCt                                              <  1:934992/139‑1 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCgg                                                                                                               <  1:660913/71‑1 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCgg                                                                                                               >  2:660913/1‑71 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGAc                                                                                                  <  2:6978/84‑1 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGAc                                                                                                  >  1:6978/1‑84 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                <  1:1115229/134‑1 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                >  2:1115229/1‑134 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                           >  1:265684/1‑139 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                           >  1:1210711/1‑139 (MQ=255)

                                                                                ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                           >  1:1226222/1‑139 (MQ=255)

                                                                                           cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                <  1:103261/139‑1 (MQ=255)

                                                                                                  tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGt                                                                                                                  >  2:11806/1‑50 (MQ=255)

                                                                                                  tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGt                                                                                                                  <  1:11806/50‑1 (MQ=255)

                                                                                                  tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             >  2:1134581/1‑55 (MQ=255)

                                                                                                  tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             <  1:1134581/55‑1 (MQ=255)

                                                                                                  tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                >  2:1242860/1‑132 (MQ=255)

                                                                                                  tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                <  1:1242860/132‑1 (MQ=255)

                                                                                                      tGAAAATGGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             <  1:879028/51‑1 (MQ=255)

                                                                                                      tGAAAATAGACGTTCATCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             >  2:879028/1‑51 (MQ=255)

                                                                                                       gAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCa                    <  1:237513/139‑1 (MQ=255)

                                                                                                        aaaaTAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAg                   <  2:265684/139‑1 (MQ=255)

                                                                                                         aaaTAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAgg                  <  1:305426/139‑1 (MQ=255)

                                                                                                              gACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             <  1:798584/43‑1 (MQ=255)

                                                                                                              gACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                             >  2:798584/1‑43 (MQ=255)

                                                                                                               aCGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaa                                                                             <  2:36556/74‑1 (MQ=255)

                                                                                                               aCGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaa                                                                             >  1:36556/1‑74 (MQ=255)

                                                                                                               aCGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCt            >  1:891841/1‑139 (MQ=255)

                                                                                                                  ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACa                                                                                                      >  1:850396/1‑46 (MQ=255)

                                                                                                                  ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACa                                                                                                      <  2:850396/46‑1 (MQ=255)

                                                                                                                  ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGAt         >  2:397358/1‑139 (MQ=255)

                                                                                                                   tcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATc        >  2:1314973/1‑139 (MQ=255)

                                                                                                                    cttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCg       >  1:706619/1‑139 (MQ=255)

                                                                                                                         tcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTg  >  2:145372/1‑139 (MQ=255)

                                                                                                                               ggTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                          >  1:192883/1‑77 (MQ=255)

                                                                                                                               ggTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                          <  2:192883/77‑1 (MQ=255)

                                                                                                                                 tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTg  >  1:423778/1‑131 (MQ=255)

                                                                                                                                 tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTg  <  2:423778/131‑1 (MQ=255)

ATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTG  >  NC_000913/3320199‑3320458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A