Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,333	T→C	E427E (GAA→GAG)	yhbX ←	putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,333	0	T	C	100.0%	187.4 / NA	52	E427E (GAA→GAG)	yhbX	putative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/28); total (24/28)

TCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAAAC  >  NC_000913/3320208‑3320468

tCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGt                                                                                                                            <  1:329107/139‑1 (MQ=255)

tCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGt                                                                                                                            <  1:417681/139‑1 (MQ=255)

  cGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCg                                                                                                                          <  1:937023/139‑1 (MQ=255)

         cGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCAGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAAt                                                                                                                   >  1:983075/1‑139 (MQ=255)

                           aGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 <  1:995520/139‑1 (MQ=255)

                             aaCGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGa                                                                                               <  1:88708/139‑1 (MQ=255)

                                  ggCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTg                                                                                                                              <  1:1361441/103‑1 (MQ=255)

                                  ggCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTg                                                                                                                              >  2:1361441/1‑103 (MQ=255)

                                                 aCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAACTCAAAAAc                                                                           >  1:832156/1‑139 (MQ=255)

                                                      gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaata                                                                                     <  2:411480/124‑1 (MQ=255)

                                                      gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaata                                                                                     >  1:411480/1‑124 (MQ=255)

                                                        gACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                    >  1:649761/1‑139 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGcc                                                                                                                                 >  1:747414/1‑68 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGcc                                                                                                                                 <  2:747414/68‑1 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 >  2:951942/1‑100 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 <  1:951942/100‑1 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCa                                                                                >  2:1173746/1‑117 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCa                                                                                <  1:1173746/117‑1 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                          >  2:45330/1‑139 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                          <  2:535699/139‑1 (MQ=255)

                                                                  gCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                          <  2:983075/139‑1 (MQ=255)

                                                                       ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 <  1:770199/95‑1 (MQ=255)

                                                                       ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 >  2:770199/1‑95 (MQ=255)

                                                                       ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 <  1:238938/95‑1 (MQ=255)

                                                                       ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                                 >  2:238938/1‑95 (MQ=255)

                                                                        tgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                    <  2:1121749/123‑1 (MQ=255)

                                                                        tgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                    >  1:1121749/1‑123 (MQ=255)

                                                                         ggCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTAGGCAAAATACATGACCGAGGCGCGGCGATCTATTAATAATTCAAAAACCTGACCTAGTAAACTATCTGTGTa                                                   <  2:832156/139‑1 (MQ=255)

                                                                               ccTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTaatgaat                                             >  1:479982/1‑139 (MQ=255)

                                                                                cTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTaatgaatg                                            <  2:1282779/139‑1 (MQ=255)

                                                                                  cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                          <  2:649761/139‑1 (MQ=255)

                                                                                        cTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                       >  1:378531/1‑56 (MQ=255)

                                                                                        cTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                       <  2:378531/56‑1 (MQ=255)

                                                                                          ccATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCAt                                  <  1:324007/139‑1 (MQ=255)

                                                                                          ccATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAACTGTGTAATGAATGGAGTTGTCAt                                  <  1:928359/139‑1 (MQ=255)

                                                                                             tGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGTTCTTTtaata                                                                                     <  2:688337/85‑1 (MQ=255)

                                                                                             tGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGTTCTTTtaata                                                                                     >  1:688337/1‑85 (MQ=255)

                                                                                              gAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCa                              <  1:553403/139‑1 (MQ=255)

                                                                                                         ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACa                                                                                                                >  2:343757/1‑46 (MQ=255)

                                                                                                         ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACa                                                                                                                <  1:343757/46‑1 (MQ=255)

                                                                                                           cttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCg                 >  2:1351698/1‑139 (MQ=255)

                                                                                                           cttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCg                 >  1:545635/1‑139 (MQ=255)

                                                                                                                  gTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                          >  1:82638/1‑107 (MQ=255)

                                                                                                                  gTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                          <  2:82638/107‑1 (MQ=255)

                                                                                                                      ggTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAg                                                                                                        >  1:888633/1‑41 (MQ=255)

                                                                                                                      ggTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAg                                                                                                        <  2:888633/41‑1 (MQ=255)

                                                                                                                        tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCa                                                                                <  1:1052512/63‑1 (MQ=255)

                                                                                                                        tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCa                                                                                >  2:1052512/1‑63 (MQ=255)

                                                                                                                        tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                          <  1:21498/85‑1 (MQ=255)

                                                                                                                        tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                          >  2:21498/1‑85 (MQ=255)

                                                                                                                        tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGaa    >  1:272571/1‑139 (MQ=255)

                                                                                                                          aCGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAAac  >  1:295040/1‑139 (MQ=255)

TCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAAAC  >  NC_000913/3320208‑3320468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A