Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,463	G→A	A116V (GCG→GTG)	dmlR ←	transcriptional activator of dmlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,463	0	G	A	100.0%	129.9 / NA	41	A116V (GCG→GTG)	dmlR	transcriptional activator of dmlA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/21); total (20/21)

GCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCC  >  NC_000913/1881337‑1881598

gCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCg                                                                                                                             >  2:805392/1‑139 (MQ=255)

       aaTTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGccac                                                                                                                      <  1:493595/139‑1 (MQ=255)

              ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                               <  1:805392/139‑1 (MQ=255)

              ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                               >  1:783853/1‑139 (MQ=255)

              ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                               >  1:562413/1‑139 (MQ=255)

                  aTGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgtcgtc                                                                                                           >  1:932355/1‑139 (MQ=255)

                             aaTGCGAATATCGAGATCGACAACTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAATGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAg                                                                                                >  1:775809/1‑139 (MQ=255)

                             aaTGCGAAGATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAg                                                                                                <  2:690738/139‑1 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGa                                                                                                                           >  2:167395/1‑110 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGa                                                                                                                           <  1:167395/110‑1 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              <  2:546235/139‑1 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              >  2:324456/1‑139 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              <  2:932355/139‑1 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              <  2:985183/139‑1 (MQ=255)

                               tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              >  1:985183/1‑139 (MQ=255)

                                  gAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgct                                                                                           <  1:66645/139‑1 (MQ=255)

                                   aaTATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGctgctg                                                                                          >  1:167620/1‑139 (MQ=255)

                                                       cATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATccc                                                                         >  1:139674/1‑136 (MQ=255)

                                                       cATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATccc                                                                         <  2:139674/136‑1 (MQ=255)

                                                            aCCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCa                                                                 >  2:67730/1‑139 (MQ=255)

                                                              cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCAc                                                                <  2:855505/138‑1 (MQ=255)

                                                              cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCAc                                                                >  1:855505/1‑138 (MQ=255)

                                                                 aTCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGa                                                            >  2:355515/1‑139 (MQ=255)

                                                                  tCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGAc                                                           <  2:167620/139‑1 (MQ=255)

                                                                   cGACCAGTCGGTCTTCCACATCAAATCGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACg                                                          <  1:927240/139‑1 (MQ=255)

                                                                    gACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGc                                                         <  1:355515/139‑1 (MQ=255)

                                                                     aCCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCa                                                        <  1:408555/139‑1 (MQ=255)

                                                                      ccAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGa                                                                                                                           >  2:263720/1‑71 (MQ=255)

                                                                      ccAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGa                                                                                                                           <  1:263720/71‑1 (MQ=255)

                                                                           cGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCa                                                                                                   >  1:554508/1‑90 (MQ=255)

                                                                           cGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCa                                                                                                   <  2:554508/90‑1 (MQ=255)

                                                                                   cacaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGc                                                         <  2:360772/124‑1 (MQ=255)

                                                                                   cacaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGc                                                         >  1:360772/1‑124 (MQ=255)

                                                                                      aTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAg                                                                                                                          <  2:202415/56‑1 (MQ=255)

                                                                                      aTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAg                                                                                                                          >  1:202415/1‑56 (MQ=255)

                                                                                        cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCaa                                                                                                  >  1:695230/1‑78 (MQ=255)

                                                                                        cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCaa                                                                                                  <  2:695230/78‑1 (MQ=255)

                                                                                        cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                                                                   >  2:14620/1‑109 (MQ=255)

                                                                                        cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                                                                   <  1:14620/109‑1 (MQ=255)

                                                                                                  ttCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCg                           >  1:382635/1‑139 (MQ=255)

                                                                                                                           aGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGccc  <  1:67730/139‑1 (MQ=255)

GCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCC  >  NC_000913/1881337‑1881598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A