Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,463	G→A	A116V (GCG→GTG)	dmlR ←	transcriptional activator of dmlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,463	0	G	A	100.0%	84.2 / NA	27	A116V (GCG→GTG)	dmlR	transcriptional activator of dmlA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/15); total (12/15)

TAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTT  >  NC_000913/1881347‑1881556

tAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCcaccac                                                                         <  2:999634/139‑1 (MQ=255)

    ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCg                                                                                   <  2:1310698/125‑1 (MQ=255)

    ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCg                                                                                   >  1:1310698/1‑125 (MQ=255)

         tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGccac                                                                            <  2:302019/127‑1 (MQ=255)

         tGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGccac                                                                            >  1:302019/1‑127 (MQ=255)

                   aaTGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTcc                                                                >  2:1157652/1‑129 (MQ=255)

                   aaTGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTcc                                                                <  1:1157652/129‑1 (MQ=255)

                                agaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATAc                                         >  1:1088251/1‑139 (MQ=255)

                                       cacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCAt                                  <  1:634448/139‑1 (MQ=255)

                                                tGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                         <  1:1132457/139‑1 (MQ=255)

                                                 gACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCATAGCTGCTGATAATACGCAGCATCCCCTGCGGc                        <  2:800776/139‑1 (MQ=255)

                                                    cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaa                                                                                           <  1:246996/69‑1 (MQ=255)

                                                    cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaa                                                                                           >  2:246996/1‑69 (MQ=255)

                                                                tCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                    <  1:658070/96‑1 (MQ=255)

                                                                tCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                    >  2:658070/1‑96 (MQ=255)

                                                                          acaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGcc                                                                                    <  2:871005/54‑1 (MQ=255)

                                                                          acaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGcc                                                                                    >  1:871005/1‑54 (MQ=255)

                                                                          acaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGat                                              <  2:1065731/92‑1 (MQ=255)

                                                                          acaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGat                                              >  1:1065731/1‑92 (MQ=255)

                                                                                   cGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAAT‑ACGCCGGAGCCACCACCTGTCGTcc                                                                <  2:153622/64‑1 (MQ=255)

                                                                                   cGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAAT‑ACGCCGGAGCCACCACCTGTCGTcc                                                                >  1:153622/1‑64 (MQ=255)

                                                                                                 cGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                          >  2:1262173/1‑73 (MQ=255)

                                                                                                 cGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                          <  1:1262173/73‑1 (MQ=255)

                                                                                                   gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                    >  2:1005818/1‑61 (MQ=255)

                                                                                                   gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                    <  1:1005818/61‑1 (MQ=255)

                                                                                                   gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGtt  >  1:844857/1‑111 (MQ=255)

                                                                                                   gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGtt  <  2:844857/111‑1 (MQ=255)

TAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTT  >  NC_000913/1881347‑1881556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A