Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,463	G→A	A116V (GCG→GTG)	dmlR ←	transcriptional activator of dmlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,463	0	G	A	100.0%	139.1 / NA	43	A116V (GCG→GTG)	dmlR	transcriptional activator of dmlA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/23); total (20/23)

TTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCC  >  NC_000913/1881330‑1881598

tttGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaat                                                                                                                                    >  1:889595/1‑139 (MQ=255)

 ttGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaata                                                                                                                                   <  1:661930/139‑1 (MQ=255)

                 tAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCcaccac                                                                                                                   <  1:1016415/139‑1 (MQ=255)

                         aTGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgtcgtc                                                                                                           >  1:488869/1‑139 (MQ=255)

                               tCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAAc                                                                                                     <  2:701549/139‑1 (MQ=255)

                                 gCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCa                                                                                                   <  1:398245/139‑1 (MQ=255)

                                      tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              >  1:904049/1‑139 (MQ=255)

                                       gCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctg                                                                                             <  2:125100/139‑1 (MQ=255)

                                        cGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                            <  1:20676/139‑1 (MQ=255)

                                        cGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                            <  2:499380/139‑1 (MQ=255)

                                            tatCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGat                                                                                        >  2:108471/1‑139 (MQ=255)

                                                   aTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTcc                                                                                                          <  1:30872/114‑1 (MQ=255)

                                                   aTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTcc                                                                                                          >  2:30872/1‑114 (MQ=255)

                                                     cGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              >  2:578515/1‑124 (MQ=255)

                                                     cGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                              <  1:578515/124‑1 (MQ=255)

                                                       acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagca                                                                             >  1:808694/1‑139 (MQ=255)

                                                       acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagca                                                                             >  1:433842/1‑139 (MQ=255)

                                                         acCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATc                                                                           <  2:493871/139‑1 (MQ=255)

                                                                 tGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                                                                   <  2:488869/139‑1 (MQ=255)

                                                                     cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaata                                                                                                                                   <  1:803986/71‑1 (MQ=255)

                                                                     cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACtaata                                                                                                                                   >  2:803986/1‑71 (MQ=255)

                                                                     cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                                                                   <  1:344249/135‑1 (MQ=255)

                                                                     cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCgg                                                                   >  2:344249/1‑135 (MQ=255)

                                                                     cAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCAcc                                                               >  1:1007042/1‑139 (MQ=255)

                                                                       aaTCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGc                                                                                                                               <  1:114923/73‑1 (MQ=255)

                                                                       aaTCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGc                                                                                                                               >  2:114923/1‑73 (MQ=255)

                                                                           gACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGc                                                         <  1:623769/139‑1 (MQ=255)

                                                                             ccAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCAc                                                       >  1:804698/1‑139 (MQ=255)

                                                                                  cGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAg                                                  >  1:629082/1‑139 (MQ=255)

                                                                                        tCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGtt                                            <  1:108471/139‑1 (MQ=255)

                                                                                           acaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGt                                         >  1:511404/1‑139 (MQ=255)

                                                                                               cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGc                                                         >  2:846079/1‑119 (MQ=255)

                                                                                               cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGc                                                         <  1:846079/119‑1 (MQ=255)

                                                                                               cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCcat                                     >  1:640302/1‑139 (MQ=255)

                                                                                                    cGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCca                                      <  2:963163/133‑1 (MQ=255)

                                                                                                    cGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCca                                      >  1:963163/1‑133 (MQ=255)

                                                                                                    cGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCt                                <  2:511404/139‑1 (MQ=255)

                                                                                                            cAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACa                        <  1:802658/139‑1 (MQ=255)

                                                                                                               ttGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATcc                     >  2:584822/1‑139 (MQ=255)

                                                                                                                tGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                            <  1:91993/67‑1 (MQ=255)

                                                                                                                tGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgctgc                                                                                            >  2:91993/1‑67 (MQ=255)

                                                                                                                                ccAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTgcgc    <  2:458048/139‑1 (MQ=255)

                                                                                                                                  aGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGccc  <  2:629082/139‑1 (MQ=255)

TTTGCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCCC  >  NC_000913/1881330‑1881598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A