Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,881,463	G→A	A116V (GCG→GTG)	dmlR ←	transcriptional activator of dmlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,881,463	0	G	A	100.0%	140.5 / NA	44	A116V (GCG→GTG)	dmlR	transcriptional activator of dmlA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/19); total (25/19)

GCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCC  >  NC_000913/1881333‑1881597

gCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAAc                                                                                                                                >  1:163714/1‑139 (MQ=255)

                  ggCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                              >  2:171550/1‑139 (MQ=255)

                      aTGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgtcgtc                                                                                                          >  2:754783/1‑139 (MQ=255)

                      aTGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgtcgtc                                                                                                          >  1:524826/1‑139 (MQ=255)

                           aTCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCaaaa                                                                                                     >  2:796382/1‑139 (MQ=255)

                           aTCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCaaaa                                                                                                     >  2:408289/1‑139 (MQ=255)

                              gCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCa                                                                                                  <  2:63943/139‑1 (MQ=255)

                                  aTGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgc                                                                                              <  1:787220/139‑1 (MQ=255)

                                   tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGc                                                                                                                        >  2:7051/1‑112 (MQ=255)

                                   tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGc                                                                                                                        <  1:7051/112‑1 (MQ=255)

                                   tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                              >  1:1229974/1‑122 (MQ=255)

                                   tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                              <  2:1229974/122‑1 (MQ=255)

                                   tGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAgct                                                                                             >  1:979981/1‑139 (MQ=255)

                                        atatCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCg                                                                                                                            >  2:91991/1‑103 (MQ=255)

                                        atatCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCg                                                                                                                            <  1:91991/103‑1 (MQ=255)

                                          atCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGata                                                                                      <  2:534073/139‑1 (MQ=255)

                                             gagaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                                                                   <  1:1137981/139‑1 (MQ=255)

                                             gagaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGataata                                                                                   <  1:728212/139‑1 (MQ=255)

                                              agaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATAc                                                                                  >  2:737813/1‑139 (MQ=255)

                                              agaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATAc                                                                                  <  2:1280797/139‑1 (MQ=255)

                                               gaTCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACg                                                                                 <  2:979981/139‑1 (MQ=255)

                                                   gACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagc                                                                             <  2:163714/139‑1 (MQ=255)

                                                    acacCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagca                                                                            >  1:1393853/1‑139 (MQ=255)

                                                        cTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATccc                                                                        >  2:1596056/1‑139 (MQ=255)

                                                           cATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTg                                                                     >  1:1326267/1‑139 (MQ=255)

                                                                    aaTCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTg                                                            >  1:456791/1‑139 (MQ=255)

                                                                                    ttCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGt                                            >  1:529788/1‑139 (MQ=255)

                                                                                       cacaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagc                                                                             <  1:899291/103‑1 (MQ=255)

                                                                                       cacaTCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACgcagc                                                                             >  2:899291/1‑103 (MQ=255)

                                                                                            cAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCcat                                    <  2:518933/139‑1 (MQ=255)

                                                                                              aaaCGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCcatca                                  >  2:1438186/1‑139 (MQ=255)

                                                                                                 cGTAATTCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCt                               >  1:270513/1‑139 (MQ=255)

                                                                                                      ttCCAGTTGCGGATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCg                          >  2:1109337/1‑139 (MQ=255)

                                                                                                                 gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                              >  1:167430/1‑44 (MQ=255)

                                                                                                                 gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTgt                                                                                                              <  2:167430/44‑1 (MQ=255)

                                                                                                                 gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCa                                                       <  2:314632/99‑1 (MQ=255)

                                                                                                                 gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCa                                                       >  1:314632/1‑99 (MQ=255)

                                                                                                                 gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCaa               >  1:1156461/1‑139 (MQ=255)

                                                                                                                 gATACGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCaa               >  1:1083294/1‑139 (MQ=255)

                                                                                                                    aCGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATcccc                                                                       <  1:934825/80‑1 (MQ=255)

                                                                                                                    aCGCCTTCGCCAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATcccc                                                                       >  2:934825/1‑80 (MQ=255)

                                                                                                                             ccAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTgcgc   <  2:1326267/139‑1 (MQ=255)

                                                                                                                              cAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGcc  <  1:408289/139‑1 (MQ=255)

                                                                                                                              cAGCACTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGcc  <  2:206493/139‑1 (MQ=255)

GCGGGCGATTAAATTAGGGGCGATGTCATCGCCAATGCGAATATCGAGATCGACACCTTCATTGACCAAATCGACCAGTCGGTCTTCCACATCAAAACGTAATTCCAGTTGCGGATACGCCTTCGCCAGCGCTAATAACGCCGGAGCCACCACCTGTCGTCCAAAACCAAAGCTGCTGATAATACGCAGCATCCCCTGCGGCACCTGACGCACGTCAGAAAGTTCGTCCATCATCTGACCGACATCCTGCAAAATCCGCTGCGCC  >  NC_000913/1881333‑1881597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A