Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,585,322 |
C→T |
T480T (ACG→ACA) |
hsdR ← |
endonuclease R Type I restriction enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,585,322 | 0 | C | T | 100.0%
| 90.0
/ NA
| 29 | T480T (ACG→ACA) | hsdR | endonuclease R Type I restriction enzyme |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/15); total (14/15) |
GTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCA > NC_000913/4585202‑4585443
|
gTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTtaccggta < 2:297375/139‑1 (MQ=255)
aaTACGCGTATCTTCAAACGCGCCCAGCGCCTGTTAGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTtaccggtaccg > 2:986383/1‑139 (MQ=255)
cgcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAt > 1:451716/1‑139 (MQ=255)
gcCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc < 1:806611/139‑1 (MQ=255)
cccAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc < 2:887885/132‑1 (MQ=255)
cccAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagc > 1:887885/1‑132 (MQ=255)
agagAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGa < 1:986383/139‑1 (MQ=255)
cggcggTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc < 1:249723/114‑1 (MQ=255)
cggcggTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGAtc > 2:249723/1‑114 (MQ=255)
aaGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGa < 1:511757/139‑1 (MQ=255)
gaATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGc < 1:881586/139‑1 (MQ=255)
aaTGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa > 2:488883/1‑139 (MQ=255)
tGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATc < 1:488883/139‑1 (MQ=255)
gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa < 1:458070/136‑1 (MQ=255)
gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCa > 2:458070/1‑136 (MQ=255)
gCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCt > 2:178829/1‑139 (MQ=255)
cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttg < 1:914575/95‑1 (MQ=255)
cGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttg > 2:914575/1‑95 (MQ=255)
gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTa < 1:905775/50‑1 (MQ=255)
gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTa > 2:905775/1‑50 (MQ=255)
gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTc > 2:427468/1‑139 (MQ=255)
cTGGATCAGGCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAgg < 2:337440/139‑1 (MQ=255)
ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca < 1:94678/60‑1 (MQ=255)
ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCcagca > 2:94678/1‑60 (MQ=255)
ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt < 2:669434/72‑1 (MQ=255)
ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCttgtt > 1:669434/1‑72 (MQ=255)
ggCGGAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctc > 1:436826/1‑139 (MQ=255)
ggAACATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAgctcgct < 1:427468/139‑1 (MQ=255)
aaCATCATGGCGATTGCTGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCCCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCa > 2:494742/1‑139 (MQ=255)
|
GTTAATACGCGTATCTTCAAACGCGCCCAGCGCCTGTTCGCCAAGAGAACGGCGGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCA > NC_000913/4585202‑4585443
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
