Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,862	A→G	D11G (GAC→GGC)	acnA →	aconitate hydratase 1, aconitase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,862	0	A	G	100.0%	234.3 / NA	66	D11G (GAC→GGC)	acnA	aconitate hydratase 1, aconitase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (39/27); total (39/27)

TCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGT  >  NC_000913/1335728‑1335995

tCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACg                                                                                                                                   >  1:1352815/1‑139 (MQ=255)

   aTTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTg                                                                                                                                <  2:106522/139‑1 (MQ=255)

    tttGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTGACCCTGTAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTCCGAGAAGCCAGTGAGGGCACGTTGc                                                                                                                               >  1:1261183/1‑139 (MQ=255)

    tttGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGc                                                                                                                               <  1:142432/139‑1 (MQ=255)

               aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGAt                                                                                                                    >  1:1069806/1‑139 (MQ=255)

                   aaTCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATaaaa                                                                                                                >  1:116523/1‑139 (MQ=255)

                          aCGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                                         <  2:1660508/139‑1 (MQ=255)

                          aCGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                                         <  1:1386930/139‑1 (MQ=255)

                                          aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCg                                                                                         >  2:1176805/1‑139 (MQ=255)

                                          aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCg                                                                                         >  2:607673/1‑139 (MQ=255)

                                               tAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgc                                                                                    <  1:607673/139‑1 (MQ=255)

                                                aaTATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgct                                                                                   >  2:414158/1‑139 (MQ=255)

                                                aaTATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgct                                                                                   >  1:943945/1‑139 (MQ=255)

                                                     tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTaa                                                                              >  1:751329/1‑139 (MQ=255)

                                                       aCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAAt                                                                            >  2:571571/1‑139 (MQ=255)

                                                       aCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAAt                                                                            >  2:296180/1‑139 (MQ=255)

                                                         ccTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          <  1:1362663/107‑1 (MQ=255)

                                                         ccTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          >  2:1362663/1‑107 (MQ=255)

                                                              agagAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGgca                                                                                                                                     >  1:488283/1‑75 (MQ=255)

                                                              agagAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGgca                                                                                                                                     <  2:488283/75‑1 (MQ=255)

                                                                  aaTCAGGCCTTCGCAACCCTGTCAATAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGat                                                                 >  1:1011996/1‑139 (MQ=255)

                                                                   aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTggg                                                                     >  2:897427/1‑134 (MQ=255)

                                                                   aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTggg                                                                     <  1:897427/134‑1 (MQ=255)

                                                                   aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGata                                                                >  1:1189615/1‑139 (MQ=255)

                                                                   aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGata                                                                >  1:963551/1‑139 (MQ=255)

                                                                       gggCTTCGCAACCCTGTCATTTAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTCGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATCTCAc                                                            <  2:963551/139‑1 (MQ=255)

                                                                       gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGc                                                                                               >  1:1259246/1‑104 (MQ=255)

                                                                       gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGc                                                                                               <  2:1259246/104‑1 (MQ=255)

                                                                       gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAc                                                            >  1:344338/1‑139 (MQ=255)

                                                                       gggCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAc                                                            >  2:1029/1‑139 (MQ=255)

                                                                          cTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                                         <  2:987103/91‑1 (MQ=255)

                                                                          cTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCa                                                                                                         >  1:987103/1‑91 (MQ=255)

                                                                           ttCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt                                                        <  1:1029/139‑1 (MQ=255)

                                                                           ttCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt                                                        <  2:344338/139‑1 (MQ=255)

                                                                            tCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          >  1:125220/1‑88 (MQ=255)

                                                                            tCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTAcc                                                                                                          <  2:125220/88‑1 (MQ=255)

                                                                                    cTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAg                                               <  1:571571/139‑1 (MQ=255)

                                                                                       tCATTAAGGAGGAGCTATGTCGTCAACACTACAAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGGCTACCCAAGTCa                                            >  2:986419/1‑139 (MQ=255)

                                                                                            aaGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCTCTGGGCGATATCAccc                                                          <  1:646051/120‑1 (MQ=255)

                                                                                            aaGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAccc                                                          >  2:646051/1‑120 (MQ=255)

                                                                                                 ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgctg                                                                                  <  1:693281/91‑1 (MQ=255)

                                                                                                 ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtgctg                                                                                  >  2:693281/1‑91 (MQ=255)

                                                                                                 ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGttt                                  >  1:899830/1‑139 (MQ=255)

                                                                                                 ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGttt                                  >  1:450959/1‑139 (MQ=255)

                                                                                                 ggagCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGttt                                  >  1:1352624/1‑139 (MQ=255)

                                                                                                   agCTATGTCGTCAACCCTACGAGAAGCCAGAAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTg                                >  1:1565897/1‑139 (MQ=255)

                                                                                                             tcAACCCTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAAcc                      >  2:878513/1‑139 (MQ=255)

                                                                                                                  ccTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      >  1:1011679/1‑86 (MQ=255)

                                                                                                                  ccTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTgg                                                                      <  2:1011679/86‑1 (MQ=255)

                                                                                                                  ccTACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACctgctg                 >  2:1487046/1‑139 (MQ=255)

                                                                                                                    tACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCtgctgc                >  2:1635466/1‑138 (MQ=255)

                                                                                                                    tACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCtgctgc                <  1:1635466/138‑1 (MQ=255)

                                                                                                                    tACGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcg               >  2:1498587/1‑139 (MQ=255)

                                                                                                                     aCGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTgcgc              <  1:1487046/139‑1 (MQ=255)

                                                                                                                      cGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             >  1:900049/1‑43 (MQ=255)

                                                                                                                      cGAGAAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACtt                                                                                                             <  2:900049/43‑1 (MQ=255)

                                                                                                                       gagaAGCCAGTAAGGGCACGTTGCAGGCCAAAGATa                                                                                                                   <  2:182446/36‑1 (MQ=38)

                                                                                                                       gagaAGCCAGTAAGGGCACGTTGCAGGCCAAAGATa                                                                                                                   >  1:182446/1‑36 (MQ=255)

                                                                                                                        agaAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTgg           <  2:1011996/139‑1 (MQ=255)

                                                                                                                         gaAGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGc          <  2:1189615/139‑1 (MQ=255)

                                                                                                                           aGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg        >  2:28602/1‑139 (MQ=255)

                                                                                                                           aGCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAg        >  2:326249/1‑139 (MQ=255)

                                                                                                                            gCCAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAgg       >  2:127470/1‑139 (MQ=255)

                                                                                                                             ccAGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGa      <  1:28602/139‑1 (MQ=255)

                                                                                                                               aGTAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATg    >  1:720719/1‑139 (MQ=255)

                                                                                                                                 tAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt  <  2:899830/139‑1 (MQ=255)

                                                                                                                                 tAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt  <  2:1352624/139‑1 (MQ=255)

                                                                                                                                 tAAGGGCACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGt  <  1:414158/139‑1 (MQ=255)

TCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGTCACTCAAAGTTTTGCTCGAAAACCTGCTGCGCTGGCAGGATGGT  >  NC_000913/1335728‑1335995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A