Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
760 |
67.6 |
3323148 |
92.7% |
3080558 |
109.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,370,044 |
(T)9→8 |
intergenic (+41/‑172) |
pspE → / → ycjM |
thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,370,036 | 0 | T | . | 100.0%
| 211.1
/ NA
| 52 | intergenic (+33/‑180) | pspE/ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (25/27); total (25/27) |
CAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGT > NC_000913/1369911‑1370159
|
caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 1:826251/139‑1 (MQ=255)
aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 1:662815/139‑1 (MQ=255)
gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCacac > 2:897311/1‑139 (MQ=255)
caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACAcc < 2:839538/139‑1 (MQ=255)
aaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCtt < 2:1285595/139‑1 (MQ=255)
tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt > 2:1135429/1‑118 (MQ=255)
tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt < 1:1135429/118‑1 (MQ=255)
tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 1:890803/139‑1 (MQ=255)
tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATccc > 1:245837/1‑139 (MQ=255)
tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATccc > 2:816942/1‑139 (MQ=255)
gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCCAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac > 1:1393164/1‑121 (MQ=255)
gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCCAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 2:1393164/121‑1 (MQ=255)
gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTAc > 2:1121068/1‑123 (MQ=255)
gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTAc < 1:1121068/123‑1 (MQ=255)
aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt > 1:819396/1‑139 (MQ=255)
aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTCATTTACATCCCCATAGATTATTTGCGt < 2:1285757/139‑1 (MQ=255)
aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac > 2:1191233/1‑103 (MQ=255)
aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 1:1191233/103‑1 (MQ=255)
cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt > 2:1461527/1‑139 (MQ=255)
gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt > 1:774846/1‑130 (MQ=255)
gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt < 2:774846/130‑1 (MQ=255)
gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca > 2:1468112/1‑139 (MQ=255)
agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 1:564014/96‑1 (MQ=255)
agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca > 2:564014/1‑96 (MQ=255)
agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa > 2:739871/1‑139 (MQ=255)
aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCa > 1:1518866/1‑92 (MQ=255)
aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCa < 2:1518866/92‑1 (MQ=255)
cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt < 1:586332/139‑1 (MQ=255)
cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt < 2:439991/63‑1 (MQ=255)
cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt > 1:439991/1‑63 (MQ=255)
cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca < 2:740246/69‑1 (MQ=255)
cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTca > 1:740246/1‑69 (MQ=255)
aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa < 1:362179/107‑1 (MQ=255)
aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa > 2:362179/1‑107 (MQ=255)
ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt < 1:1461527/139‑1 (MQ=255)
cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc > 2:819409/1‑139 (MQ=255)
aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCacac > 2:924173/1‑57 (MQ=255)
aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCacac < 1:924173/57‑1 (MQ=255)
gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa > 2:299390/1‑139 (MQ=255)
aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCacac < 2:1256982/51‑1 (MQ=255)
aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCacac > 1:1256982/1‑51 (MQ=255)
aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc < 1:897311/139‑1 (MQ=255)
aaaaGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc < 2:245837/139‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTAtt > 2:929684/1‑50 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTAtt < 1:929684/50‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTAc < 1:1060647/53‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTAc > 2:1060647/1‑53 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt < 2:1390062/121‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt > 1:1390062/1‑121 (MQ=255)
cggcgCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaat < 2:1563757/97‑2 (MQ=255)
cggcgCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaat > 1:1563757/1‑96 (MQ=255)
cggcgCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGt < 1:1468112/139‑1 (MQ=255)
gcgcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATcccc > 2:1166386/1‑46 (MQ=255)
gcgcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATcccc < 1:1166386/46‑1 (MQ=255)
cgcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg > 2:1094241/1‑87 (MQ=255)
cgcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg < 1:1094241/87‑1 (MQ=255)
|
CAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGT > NC_000913/1369911‑1370159
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
