Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,370,044 |
(T)9→8 |
intergenic (+41/‑172) |
pspE → / → ycjM |
thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,370,036 | 0 | T | . | 90.9%
| 138.0
/ 11.3
| 44 | intergenic (+33/‑180) | pspE/ycjM | thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base . (18/22); total (20/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
CGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAT > NC_000913/1369899‑1370163
|
cGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtt > 1:23705/1‑139 (MQ=255)
aGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt < 1:1104228/139‑1 (MQ=255)
gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttt < 1:1146782/139‑1 (MQ=255)
tCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt < 1:139846/139‑1 (MQ=255)
aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 2:23705/139‑1 (MQ=255)
agcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcaca < 2:1728421/139‑1 (MQ=255)
aaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTa < 2:780247/139‑1 (MQ=255)
gagaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt < 2:1063570/122‑1 (MQ=255)
aagaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGACAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt > 1:1063570/2‑122 (MQ=255)
tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 1:1671426/139‑1 (MQ=255)
tCCTTAGCGAGATGGGATATACCCACCTTGAGAACGCTGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 2:1103791/139‑1 (MQ=255)
ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAt < 1:1280901/139‑1 (MQ=255)
tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACa > 1:100728/1‑136 (MQ=255)
tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACa < 2:100728/136‑1 (MQ=255)
aGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACAT‑ccc < 2:512811/139‑1 (MQ=255)
cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCTGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt > 2:199031/1‑113 (MQ=255)
cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt < 1:199031/113‑1 (MQ=255)
gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGa < 2:902127/139‑1 (MQ=255)
aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac > 2:942828/1‑116 (MQ=255)
aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac < 1:942828/116‑1 (MQ=255)
aCCCACGTTGAGAACGCTGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTccc < 2:1041794/107‑3 (MQ=255)
aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTcac > 1:1041794/1‑107 (MQ=255)
aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTNNNCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGt > 1:999906/1‑139 (MQ=255)
aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttt < 2:781407/95‑1 (MQ=255)
aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttt > 1:781407/1‑95 (MQ=255)
aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCa > 2:345734/1‑121 (MQ=255)
aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCa < 1:345734/121‑1 (MQ=255)
cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGTTTAAAAGAATTCACCATGAGCGGCGCTAATGCGCCG‑TTTTTTTTCTGTca < 2:1315652/101‑1 (MQ=255)
cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttctttca > 1:1315652/1‑95 (MQ=255)
cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCt > 2:1438819/1‑139 (MQ=255)
agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa > 2:1274/1‑109 (MQ=255)
agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 1:1274/109‑1 (MQ=255)
gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGt < 1:194168/124‑1 (MQ=255)
gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGt > 2:194168/1‑124 (MQ=255)
tGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttctgt > 2:1479101/1‑80 (MQ=255)
tGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttttctgt < 1:1479101/84‑5 (MQ=255)
aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTT‑TGTCCCAACCTATTTTCCTCCCCCTTAGATT‑TTTTCCTCAGCTCTCAAATAAGCCTTTTCCCCgg > 2:780108/1‑139 (MQ=255)
cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACa < 2:683658/87‑1 (MQ=255)
cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTAGGCGCCG‑TTTTTTTTCTTTCACACCTTATTTACa > 1:683658/1‑87 (MQ=255)
aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa > 2:369053/1‑139 (MQ=255)
aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTAc > 1:1223967/1‑66 (MQ=255)
aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTAc < 2:1223967/66‑1 (MQ=255)
aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa > 2:1720888/1‑139 (MQ=255)
aaTTCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgtg < 1:1720888/139‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCt < 2:923659/79‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCt > 1:923659/1‑79 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc > 2:346446/1‑97 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc < 1:346446/97‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc > 2:853534/1‑119 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc < 1:853534/119‑1 (MQ=255)
ttCACCATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt > 1:1528395/1‑139 (MQ=255)
cATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCt > 1:296498/1‑74 (MQ=255)
cATGAGCGGCGCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCt < 2:296498/74‑1 (MQ=255)
cggcgCTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGt < 2:1528395/139‑1 (MQ=255)
gcTTATGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt > 1:992045/1‑139 (MQ=255)
tGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCa < 2:45117/40‑1 (MQ=255)
tGCGCCG‑TTTTTTTTCTGTCACACCTTATTTACAT‑CCCCa > 1:45117/1‑40 (MQ=255)
|
CGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACAT‑CCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAT > NC_000913/1369899‑1370163
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
