Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,624,357 |
(T)8→7 |
coding (141/393 nt) |
ydeI ← |
hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,624,350 | 0 | T | . | 100.0%
| 97.2
/ NA
| 23 | coding (148/393 nt) | ydeI | hydrogen peroxide resistance OB fold protein, putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (10/13); total (10/13) |
AACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACA > NC_000913/1624225‑1624480
|
aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat < 1:149165/139‑1 (MQ=255)
aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc > 2:489436/1‑139 (MQ=255)
cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTTGTGAAGCTGAAGCCCCGTCATGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg < 2:313873/139‑1 (MQ=255)
ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc < 1:320739/139‑1 (MQ=255)
ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc < 1:30487/139‑1 (MQ=255)
aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt > 2:210788/1‑139 (MQ=255)
cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg < 1:489436/139‑1 (MQ=255)
ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc < 1:176559/139‑1 (MQ=255)
tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc > 2:827703/1‑139 (MQ=255)
gaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCcg > 1:417579/1‑89 (MQ=255)
gaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCcg < 2:417579/89‑1 (MQ=255)
aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt > 1:823465/1‑139 (MQ=255)
cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc > 2:721035/1‑72 (MQ=255)
cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc < 1:721035/72‑1 (MQ=255)
aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc > 2:430914/1‑139 (MQ=255)
aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAtttttt > 1:116412/1‑45 (MQ=255)
aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAtttttt < 2:116412/45‑1 (MQ=255)
aTCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCa > 1:837097/1‑139 (MQ=255)
tCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt < 1:827703/139‑1 (MQ=255)
tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa > 2:644065/1‑139 (MQ=255)
tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa < 1:430914/139‑1 (MQ=255)
tGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTg < 1:456340/139‑1 (MQ=255)
aTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa > 1:876829/1‑136 (MQ=255)
aTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa < 2:876829/136‑1 (MQ=255)
cAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa < 1:210788/139‑1 (MQ=255)
|
AACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACA > NC_000913/1624225‑1624480
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
