Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,624,357	(T)_8→7	coding (141/393 nt)	ydeI ←	hydrogen peroxide resistance OB fold protein, putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,624,350	0	T	.	97.0%	265.0 / 1.6	67	coding (148/393 nt)	ydeI	hydrogen peroxide resistance OB fold protein, putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (28/37); minor base C (1/1); total (29/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGG‑TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACA  >  NC_000913/1624214‑1624480

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAtt                                                                                                                                   >  1:895875/1‑139 (MQ=255)

 ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAttt                                                                                                                                  >  2:107259/1‑139 (MQ=255)

       gAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                            <  2:513871/139‑3 (MQ=255)

                  ttAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc                                                                                                                >  1:688548/1‑139 (MQ=255)

                          aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                        <  2:981179/139‑1 (MQ=255)

                          aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                        <  2:897230/139‑1 (MQ=255)

                          aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                        <  2:688548/139‑1 (MQ=255)

                            cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                      <  2:1619005/139‑1 (MQ=255)

                               tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                   <  2:976596/139‑1 (MQ=255)

                               tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                   <  2:895875/139‑1 (MQ=255)

                               tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtc                                                                                                   <  2:273178/139‑1 (MQ=255)

                                ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAtct                                                                                                  >  2:2049978/1‑139 (MQ=255)

                                   aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                      <  2:142039/132‑1 (MQ=255)

                                   aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                      >  1:142039/1‑132 (MQ=255)

                                           aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCACTTTTTTTGCATCATCGGt                                                                                                                  >  1:310183/1‑113 (MQ=255)

                                           aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCACTTTTTTTGCATCATCGGt                                                                                                                  <  2:310183/113‑1 (MQ=255)

                                               gTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                   <  1:1341416/139‑1 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                    <  2:28222/115‑1 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCAt                                                                                                    >  1:28222/1‑115 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg                                                                            <  2:771290/139‑1 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg                                                                            >  1:881647/1‑139 (MQ=255)

                                                         tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                            <  2:1636264/89‑3 (MQ=255)

                                                         tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                            <  2:1329269/89‑3 (MQ=255)

                                                         tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                            >  1:1636264/1‑87 (MQ=255)

                                                         tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                            >  1:1329269/1‑87 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                  <  2:313720/139‑1 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                  >  1:1839468/1‑139 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                  <  2:1839468/139‑1 (MQ=255)

                                                                    agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                       >  2:1362950/1‑130 (MQ=255)

                                                                    agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                       <  1:1362950/130‑1 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                      >  2:1381236/1‑80 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                      <  1:1381236/80‑1 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGAt                                                                                                          >  2:649330/1‑92 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGAt                                                                                                          <  1:649330/92‑1 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtttt                                                           >  1:1983472/1‑139 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtttt                                                           >  1:15430/1‑139 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGtttt                                                           >  2:1920412/1‑139 (MQ=255)

                                                                        aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                          <  2:15430/139‑1 (MQ=255)

                                                                               tACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGcc                                                   <  1:1920412/139‑1 (MQ=255)

                                                                                 ccGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                 >  1:328282/1‑139 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                        <  2:1815547/81‑1 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAg                                                                                                        >  1:1815547/1‑81 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                              >  2:121922/1‑139 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                              >  1:1242378/1‑139 (MQ=255)

                                                                                           aaaCCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa                                                                                      >  2:1238443/1‑92 (MQ=255)

                                                                                           aaaCCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa                                                                                      <  1:1238443/92‑1 (MQ=255)

                                                                                             aCCGAAGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                     <  2:328282/139‑1 (MQ=255)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAttttttt                                                                                                                              >  1:1042410/1‑47 (MQ=38)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCAttttttt                                                                                                                              <  2:1042410/47‑1 (MQ=38)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGc                                 >  1:381535/1‑139 (MQ=255)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGc                                 >  1:644116/1‑139 (MQ=255)

                                                                                                  aGCGCCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                <  2:1242378/139‑1 (MQ=255)

                                                                                                   gcgcCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                      <  1:1164281/52‑1 (MQ=37)

                                                                                                   gcgcCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                      >  2:1164281/1‑52 (MQ=37)

                                                                                                   gcgcCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCAt                               >  2:697456/1‑139 (MQ=255)

                                                                                                     gcCATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATaa                             <  1:2049978/139‑1 (MQ=255)

                                                                                                       cATCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg                           >  2:1566429/1‑139 (MQ=255)

                                                                                                        aTCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                  >  2:95101/1‑99 (MQ=255)

                                                                                                        aTCGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                  <  1:95101/99‑1 (MQ=255)

                                                                                                          cGTGCATGGTTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                        <  2:881647/139‑1 (MQ=255)

                                                                                                                  gttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                <  1:1566429/137‑1 (MQ=255)

                                                                                                                  gttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                <  2:436314/137‑1 (MQ=255)

                                                                                                                   ttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATg                                                                                       <  1:586436/66‑1 (MQ=255)

                                                                                                                   ttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATg                                                                                       >  2:586436/2‑67 (MQ=255)

                                                                                                                   ttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCAt                                           >  2:390153/2‑111 (MQ=255)

                                                                                                                   ttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCAt                                           <  1:390153/110‑1 (MQ=255)

                                                                                                                   ttttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaa               >  1:547551/2‑139 (MQ=255)

                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa              <  2:1983472/139‑1 (MQ=255)

                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa              <  1:121922/139‑1 (MQ=255)

                                                                                                                    tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa              <  2:1970960/139‑1 (MQ=255)

                                                                                                                      tttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                <  2:793963/135‑1 (MQ=255)

                                                                                                                      tttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                >  1:793963/1‑135 (MQ=255)

                                                                                                                           gAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa       <  2:547551/139‑1 (MQ=255)

                                                                                                                             aaTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                        >  1:1842025/1‑88 (MQ=255)

                                                                                                                             aaTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                        <  2:1842025/88‑1 (MQ=255)

                                                                                                                                cAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa  <  1:107259/139‑1 (MQ=255)

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGG‑TTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACA  >  NC_000913/1624214‑1624480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A